Growth Hormone 1 antibody (AA 27-216)

Catalog No. ABIN3042811

Product Details anti-Growth Hormone 1 Antibody

Target: Growth Hormone 1 (GH1)

Binding Specificity: AA 27-216

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Growth Hormone 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Somatotropin(GH1) detection. Tested with WB, IHC-P, ELISA in Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Somatotropin(GH1) detection. Tested with WB, IHC-P, ELISA in Rat.
Gene Name: growth hormone 1
Protein Name: Somatotropin

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived rat GH recombinant protein(Position: F27-F216).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Species: Rat
IHC-P: Concentration: 0.5-1 μg/mL, Species: Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Species: Rat

Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Growth Hormone 1

Target: Growth Hormone 1 (GH1)

Alternative Name: GH1 (GH1 Products)

Synonyms: GH antibody, GH-N antibody, GHN antibody, IGHD1B antibody, hGH-N antibody, Gh1 antibody, GH1 antibody, gh antibody, Gh antibody, GH2 antibody, RNGHGP antibody, ghl antibody, gh-n antibody, ghn antibody, ighd1b antibody, gh1 antibody, ghb-A antibody, GHI antibody, GHB3 antibody, growth hormone 1 antibody, growth hormone antibody, somatotropin-like antibody, somatotropin antibody, growth hormone prepeptide antibody, growth hormone 1 L homeolog antibody, growth hormone 1 S homeolog antibody, Somatotropin-1 antibody, GH1 antibody, Gh antibody, GH antibody, LOC100305005 antibody, Gh1 antibody, LOC100534452 antibody, LOC100232594 antibody, LOC100303681 antibody, gh1 antibody, LOC100356068 antibody, LOC100136588 antibody, gh1.L antibody, gh1.S antibody, LOC109081196 antibody

Background: Growth Hormone(GH) is mapped to 17q22-q24. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. Rat GH shares 98 % amino acid sequence homology with mouse. It binds two receptor molecules and thereby induces signal transduction through receptor dimerization. At high concentrations, GH acts as an antagonist because of a large difference in affinities at the respective binding sites.

Synonyms: gH antibody|GH-N antibody|GH1 antibody|Growth hormone 1 antibody|Growth hormone antibody|hGH-N antibody|IGHD1B antibody|Pituitary growth hormone antibody|Somatotropin antibody

UniProt: P01244

Pathways: NF-kappaB Signaling, JAK-STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process