COL2A1 antibody (C-Term)

Catalog No. ABIN3042879

Product Details anti-COL2A1 Antibody

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Binding Specificity: AA 1222-1241, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL2A1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Collagen alpha-1(II) chain(COL2A1) detection. Tested with WB in Human,Mouse,Rat.

Sequence: AFAGLGPREK GPDPLQYMRA

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Collagen alpha-1(II) chain(COL2A1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: collagen, type II, alpha 1
Protein Name: Collagen alpha-1(II) chain

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen II(1222-1241aa AFAGLGPREKGPDPLQYMRA), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-COL2A1 antibody (ABIN3042879)

Jia, Ma, Lv, Ma, Xu, Yang, Tian, Wang, Sun, Xu, Fu, Zhao: "Oestrogen and parathyroid hormone alleviate lumbar intervertebral disc degeneration in ovariectomized rats and enhance Wnt/β-catenin pathway activity." in: Scientific reports, Vol. 6, pp. 27521, (2018) (PubMed).

Qin, Jin, Jiang, Li, Tan, Wu, Zheng, Zhao: "A Human Chondrocyte-Derived In Vitro Model of Alcohol-Induced and Steroid-Induced Femoral Head Necrosis." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 539-547, (2018) (PubMed).

Jiang, Zhong, Zheng, Zhao: "Nano-hydroxyapatite/collagen film as a favorable substrate to maintain the phenotype and promote the growth of chondrocytes cultured in vitro." in: International journal of molecular medicine, Vol. 41, Issue 4, pp. 2150-2158, (2018) (PubMed).

Huang, Wu, Wang, Zheng, Zhao: "Protective effects of baicalin on rabbit articular chondrocytes in vitro." in: Experimental and therapeutic medicine, Vol. 13, Issue 4, pp. 1267-1274, (2017) (PubMed).

Tan, Lu, Miao, Lei, Zheng, Zhao: "Effect of NGF From Venom of Chinese Cobra (Naja Atra)on Chondrocytes Proliferation and Metabolism In Vitro." in: Journal of cellular biochemistry, (2017) (PubMed).

Hou, Huang, Luo, Wang, Liu, Deng, Zhang, Liu, Chen: "MiR-351 negatively regulates osteoblast differentiation of MSCs induced by (+)-cholesten-3-one through targeting VDR." in: American journal of translational research, Vol. 9, Issue 11, pp. 4963-4973, (2017) (PubMed).

Tian, Zhong, Zheng, Zhang, Liu, Zhang, Liu: "Neuroleukin/Autocrine Motility Factor Receptor Pathway Promotes Proliferation of Articular Chondrocytes through Activation of AKT and Smad2/3." in: Scientific reports, Vol. 5, pp. 15101, (2016) (PubMed).

Liu, Tian, Zhou, Wang, Gou, Zhang, Wang, Shen, Zhang, Zhang: "Protective effect of calcitonin on lumbar fusion-induced adjacent-segment disc degeneration in ovariectomized rat." in: BMC musculoskeletal disorders, Vol. 16, pp. 342, (2016) (PubMed).

Tian, Zhong, Zhang, Yin, Zhang, Liu: "Microfluidics‑based optimization of neuroleukin‑mediated regulation of articular chondrocyte proliferation." in: Molecular medicine reports, Vol. 13, Issue 1, pp. 67-74, (2016) (PubMed).

Gu, Li, Li, Yu, Wang, Ding, Liu: "Oral Resveratrol Prevents Osteoarthritis Progression in C57BL/6J Mice Fed a High-Fat Diet." in: Nutrients, Vol. 8, Issue 4, pp. 233, (2016) (PubMed).

Li, Sun, Liu, Chen, Rong, Lou, Yang, Yang, Liu: "Use of a biological reactor and platelet-rich plasma for the construction of tissue-engineered bone to repair articular cartilage defects." in: Experimental and therapeutic medicine, Vol. 12, Issue 2, pp. 711-719, (2016) (PubMed).

Liu, Lu, Wu, Zheng: "Chondroprotective effects of taurine in primary cultures of human articular chondrocytes." in: The Tohoku journal of experimental medicine, Vol. 235, Issue 3, pp. 201-13, (2015) (PubMed).

Ye, Wu, Li, Li, Zheng, Liu, Ye: "Millimeter Wave Treatment Inhibits Apoptosis of Chondrocytes via Regulation Dynamic Equilibrium of Intracellular Free Ca (2+)." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 464161, (2015) (PubMed).

Cai, Zhang, Zhang, Ge, Dai, Tan, Roodrajeetsing, Cai: "Promoting Effects on Proliferation and Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells by Four "Kidney-Tonifying" Traditional Chinese Herbs." in: BioMed research international, Vol. 2015, pp. 792161, (2015) (PubMed).

Sun, Meng, Li, Liu, Chen: "Construction of tissue-engineered laryngeal cartilage with a hollow, semi-flared shape using poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) as a scaffold." in: Experimental and therapeutic medicine, Vol. 9, Issue 4, pp. 1482-1488, (2015) (PubMed).

Wang, Yao, Huang, Li, Xu: "Effects of TGF-β1 and alginate on the differentiation of rabbit bone marrow-derived mesenchymal stem cells into a chondrocyte cell lineage." in: Experimental and therapeutic medicine, Vol. 10, Issue 3, pp. 995-1002, (2015) (PubMed).

Ouyang, Wang, Hong, Xin, Wang, Yang, Wang, Wang, Wei, Wang, Cui: "Establishment of a rat model for osteoarthritis resulting from anterior cruciate ligament rupture and its significance." in: Experimental and therapeutic medicine, Vol. 10, Issue 6, pp. 2035-2038, (2015) (PubMed).

Liu, Liu, Lin, Wei, Zheng: "Effect of JEZTC, a synthetic compound, on proliferation and phenotype maintenance of rabbit articular chondrocytes in vitro." in: In vitro cellular & developmental biology. Animal, Vol. 50, Issue 10, pp. 982-91, (2014) (PubMed).

Ye, Hao, Song, Zhao, Fang, Wang, Li: "Microenvironment is involved in cellular response to hydrostatic pressures during chondrogenesis of mesenchymal stem cells." in: Journal of cellular biochemistry, Vol. 115, Issue 6, pp. 1089-96, (2014) (PubMed).

Zhang, Zhang, Shi, Tan, Han, Ye, Pan, Sun, Liu: "Comparisons of rabbit bone marrow mesenchymal stem cell isolation and culture methods in vitro." in: PLoS ONE, Vol. 9, Issue 2, pp. e88794, (2014) (PubMed).

Target Details for COL2A1

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Alternative Name: COL2A1 (COL2A1 Products)

Synonyms: ANFH antibody, AOM antibody, COL11A3 antibody, SEDC antibody, Col2 antibody, Col2a antibody, Col2a-1 antibody, Del1 antibody, Dmm antibody, Lpk antibody, M100856 antibody, Rgsc856 antibody, CG2A1A antibody, COLLII antibody, col2a1a antibody, CB11 antibody, col2a1 antibody, coll2a1 antibody, fb38c06 antibody, fc10c01 antibody, wu:fb38c06 antibody, wu:fc10c01 antibody, collagen type II alpha 1 chain antibody, collagen, type II, alpha 1 antibody, collagen, type II, alpha 1 L homeolog antibody, collagen, type II, alpha 1 S homeolog antibody, collagen, type II, alpha 1a antibody, COL2A1 antibody, Col2a1 antibody, col2a1 antibody, col2a1.L antibody, col2a1.S antibody, col2a1a antibody

Background: Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Synonyms: Alpha 1 type II collagen antibody|Alpha-1 type II collagen antibody|AOM antibody|Cartilage collagen antibody|Chondrocalcin antibody|CO2A1_HUMAN antibody|COL11A3 antibody|COL2A1 antibody|COL2A1 protein antibody|Collagen alpha 1(II) chain precursor antibody|Collagen II alpha 1 polypeptide antibody|Collagen type II alpha 1(primary osteoarthritis spondyloepiphyseal dysplasia congenital) antibody|MGC131516 antibody|SEDC antibody

UniProt: P02458

Pathways: Sensory Perception of Sound, Growth Factor Binding