Aquaporin 10 antibody (C-Term)

Catalog No. ABIN3042903

Product Details anti-Aquaporin 10 Antibody

Target: Aquaporin 10 (AQP10)

Binding Specificity: AA 286-301, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 10 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Aquaporin-10(AQP10) detection. Tested with WB in Human.

Sequence: SELETPASAQ MLECKL

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Aquaporin-10(AQP10) detection. Tested with WB in Human.
Gene Name: aquaporin 10
Protein Name: Aquaporin-10

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 10(286-301aa SELETPASAQMLECKL).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for AQP10 is approximately 3 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Aquaporin 10

Target: Aquaporin 10 (AQP10)

Alternative Name: AQP10 (AQP10 Products)

Synonyms: aqp10 antibody, zgc:92167 antibody, AQP10 antibody, AQPA_HUMAN antibody, aquaporin 10a antibody, aquaporin 10 antibody, similar to aquaporin 10 antibody, aqp10a antibody, AQP10 antibody, LOC686596 antibody

Background: AQP10 was identified in human small intestine. This gene encoded a 264-amino-acid protein with high sequence identity with AQP3(53 % ), 9(52 %), and 7(43 %). These AQPs constitute one subfamily of AQP family that is differentiated from the other subfamily of AQP(AQP0, 1, 2, 4, 5, 6, and 8) by sequence homology. Northern blot analysis revealed expression of a 2.3-kb AQP10 transcript in jejunum but not liver. RNase protection analysis detected nearly exclusive expression in duodenum and jejunum. In situ hybridization analysis demonstrated highest expression of AQP10 in absorptive epithelial cells at the tips of villi in the jejunum. SDS-PAGE analysis showed expression of a 28-kD AQP10 protein, similar to its predicted size as well as to that of the other AQPs. Functional analysis indicated that cells expressing AQP10 are permeable to relatively low amounts of water, but, unlike AQP3, AQP7, and AQP9, are not permeable to urea or glycerol.

Synonyms: AQP 10 antibody|AQP10 antibody|AQPA Human antibody|Aquaporin10 antibody|Small intestine aquaporin antibody