Flotillin 2 antibody (N-Term)
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- Target See all Flotillin 2 (FLOT2) Antibodies
- Flotillin 2 (FLOT2)
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Binding Specificity
- AA 117-131, N-Term
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Reactivity
- Human, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Flotillin 2 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Purpose
- Rabbit IgG polyclonal antibody for Flotillin-2(FLOT2) detection. Tested with WB in Human,Mouse,Rat.
- Sequence
- TLTVEQIYQD RDQFA
- Cross-Reactivity (Details)
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Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - Characteristics
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Rabbit IgG polyclonal antibody for Flotillin-2(FLOT2) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: flotillin 2
Protein Name: Flotillin-2 - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the N-terminus of human Flotillin 2(117-131aa TLTVEQIYQDRDQFA), identical to the related rat and mouse sequences.
- Isotype
- IgG
- Top Product
- Discover our top product FLOT2 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for FLOT2 is approximately 1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Thimerosal (Merthiolate), Sodium azide
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Expiry Date
- 12 months
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- Target
- Flotillin 2 (FLOT2)
- Alternative Name
- FLOT2 (FLOT2 Products)
- Synonyms
- ECS-1 antibody, ECS1 antibody, ESA antibody, ESA1 antibody, M17S1 antibody, AI573412 antibody, Esa antibody, reggie-2 antibody, CG11547 antibody, CG14409 antibody, CG32593 antibody, DmFlo2 antibody, Dmel\\CG32593 antibody, FLO2 antibody, FLO[Dm]-2 antibody, Flo2 antibody, flo2 antibody, reggie-1 antibody, ecs-1 antibody, ecs1 antibody, esa antibody, esa1 antibody, flotillin-2 antibody, FLOT2 antibody, fb48a04 antibody, im:7143869 antibody, wu:fb48a04 antibody, zgc:55821 antibody, flotillin 2 antibody, Flotillin 2 antibody, flotillin 2 L homeolog antibody, flotillin 2a antibody, FLOT2 antibody, Flot2 antibody, Flo2 antibody, flot2.L antibody, flot2 antibody, flot2a antibody
- Background
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FLOT2(Flotillin 2), also known as ESA1 or M17S1, is a protein that in humans is encoded by the FLOT2 gene. Schroeder et al.(1991) isolated a cDNA for an epidermal surface antigen believed to be involved in epidermal cell adhesion. By analysis of a somatic cell hybrid panel and in situ hybridization using the ESA cDNA, the gene was mapped to 17q11-q12 in the region containing the NF1 gene. Bickel et al.(1997) found that mouse Flot2 consistently copurifies with Flot1 and with caveolin-1 in the purification of caveolin-rich membranes. Using a quantitative proteomic analysis of cultured mouse neuronal stem cells, Li et al.(2012) found that palmitoylation and oligomerization of flotillin-2 was abolished in homozygous Dhhc5 mutant neuronal stem cells. The absolute amount of flotillin-2 was not changed in Dhhc5 mutant neurons.
Synonyms: ECS-1 antibody|ECS1 antibody|Epidermal surface antigen 1 antibody|Epidermal surface antigen antibody|ESA antibody|ESA1 antibody|Flot2 antibody|FLOT2_HUMAN antibody|Flotillin-2 antibody|M17S1 antibody|Membrane component chromosome 17 surface marker 1 antibody|Membrane component chromosome 17 surface marker 1 homolog antibody|Membrane component, chromosome 17, surface marker 1 antibody|REG-1 antibody|Reggie-1 antibody|Reggie-2 antibody - UniProt
- Q14254
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