Flotillin 1 antibody (Middle Region)

Catalog No. ABIN3042941

Product Details anti-Flotillin 1 Antibody

Target: Flotillin 1 (FLOT1)

Binding Specificity: AA 219-234, Middle Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Flotillin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Flotillin-1(FLOT1) detection. Tested with WB in Human,Mouse,Rat.

Sequence: KKAAYDIEVN TRRAQA

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Flotillin-1(FLOT1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: flotillin 1
Protein Name: Flotillin-1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Flotillin 1(219-234aa KKAAYDIEVNTRRAQA), different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for FLOT1 is approximately 0.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-Flotillin 1 antibody (ABIN3042941)

Shi, Li, Liu, Yin, Zhao: "β-Cyclodextrin induces the differentiation of resident cardiac stem cells to cardiomyocytes through autophagy." in: Biochimica et biophysica acta. Molecular cell research, Vol. 1864, Issue 8, pp. 1425-1434, (2017) (PubMed).

Target Details for Flotillin 1

Target: Flotillin 1 (FLOT1)

Alternative Name: FLOT1 (FLOT1 Products)

Synonyms: reggie-2 antibody, flot1 antibody, Rareg antibody, flot1c antibody, flotillin-1 antibody, flotillin1 antibody, flot1a antibody, reggie2 antibody, si:dz44o19.1 antibody, wu:fj45g11 antibody, flotillin 1 antibody, flotillin-1 antibody, putative flotillin-1 antibody, flotillin 1 S homeolog antibody, flotillin 1 L homeolog antibody, flotillin 1a antibody, FLOT1 antibody, Flot1 antibody, flot1 antibody, LOC5566722 antibody, CpipJ_CPIJ008623 antibody, Smp_016200.1 antibody, flot1.S antibody, flot1.L antibody, flot1a antibody

Background: FLOT1(Flotillin 1), is a protein that in humans is encoded by the FLOT1 gene. The International Radiation Hybrid Mapping Consortium mapped the FLOT1 gene to chromosome 6. Bickel et al.(1997) found that mouse Flot1 behaves as a resident integral membrane protein of caveolae. It consistently copurified with Flot2 and with caveolin-1 in the purification of caveolin-rich membranes. Hazarika et al.(1999) found that stable transfection of Flot1, which they called ESA/flotillin-2, in COS-1 cells induced filopodia formation and changed the epithelial morphology to that of neuronal cells. Santamaria et al.(2005) found that prostate tumor overexpressed gene-1 interacted with flotillin-1 in detergent-insoluble membrane fractions. Flotillin-1 colocalized with PTOV1 at the plasma membrane and in the nucleus, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase.

Synonyms: FLOT 1 antibody|FLOT1 antibody|FLOT1_HUMAN antibody|Flotillin-1 antibody|Flotillin1 antibody|Integral membrane component of caveolae antibody|Reggie 2 antibody

UniProt: O75955