VGF antibody (C-Term)

Catalog No. ABIN3042968

Product Details anti-VGF Antibody

Target: VGF (VGF Nerve Growth Factor Inducible (VGF))

Binding Specificity: AA 582-600, C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VGF antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Neurosecretory protein VGF(VGF) detection. Tested with WB in Human,Mouse,Rat.

Sequence: QARRAQEEAE AEERRLQEQ

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Neurosecretory protein VGF(VGF) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: VGF nerve growth factor inducible
Protein Name: Neurosecretory protein VGF

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human VGF(582-600aa QARRAQEEAEAEERRLQEQ), different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for VGF is approximately 0.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for VGF

Target: VGF (VGF Nerve Growth Factor Inducible (VGF))

Alternative Name: VGF (VGF Products)

Synonyms: VGF antibody, Gm1052 antibody, VGF nerve growth factor inducible antibody, VGF antibody, Vgf antibody

Background: VGF(VGF, Nerve Growth Factor-Inducible), is a protein and neuropeptide that may play a role in regulating energy homeostasis, metabolism. Canu et al.(1997) noted that rat Vgf encodes a predicted 70-kD polypeptide that shares similarities with the secretogranin/chromogranin family and is found in the secretory granules of subsets of neurons and endocrine cells. By fluorescence in situ hybridization, Canu et al.(1997) assigned the VGF gene to 7q22. Canu et al.(1997) demonstrated that the single-copy human VGF gene spans 6 kb of genomic DNA and contains 2 exons. The entire VGF protein is encoded by exon 2, while exon 1 contains only 5-prime untranslated sequence. The structural organization of the human gene is similar to that described for the rat VGF gene(Salton et al., 1991), and both the translated and the untranslated regions show a high degree of sequence homology to the rat gene.

Synonyms: NERP-1 antibody|NERP-2 antibody|Nerve growth factor inducible antibody|Neuroendocrine regulatory peptide-2 antibody|Neurosecretory protein VGF antibody|VGF antibody|VGF_HUMAN antibody

UniProt: O15240

Pathways: Hormone Transport, Carbohydrate Homeostasis