Caspase 4 antibody (N-Term)

Catalog No. ABIN3043186

Product Details anti-Caspase 4 Antibody

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Binding Specificity: AA 104-124, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Caspase-4(CASP4) detection. Tested with WB, IHC-P in Human.

Sequence: DALKLCPHEE FLRLCKERAE E

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Caspase-4(CASP4) detection. Tested with WB, IHC-P in Human.
Gene Name: caspase 4, apoptosis-related cysteine peptidase
Protein Name: Caspase-4

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase 4(104-124aa DALKLCPHEEFLRLCKERAEE).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-Caspase 4 antibody (ABIN3043186)

Ji, Yan, Li, Lai, Liu: "Therapeutic effects of intrathecal versus intravenous monosialoganglioside against bupivacaine-induced spinal neurotoxicity in rats." in: Biomedicine & pharmacotherapy = Biome?decine & pharmacothe?rapie, Vol. 69, pp. 311-6, (2015) (PubMed).

Chen, Wei, Xie, Wang, Cheng, Cheng: "Endoplasmic reticulum stress contributes to arsenic trioxide-induced apoptosis in drug-sensitive and -resistant leukemia cells." in: Leukemia research, Vol. 36, Issue 12, pp. 1526-35, (2012) (PubMed).

Target Details for Caspase 4

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Alternative Name: CASP4 (CASP4 Products)

Synonyms: ICE(rel)II antibody, ICEREL-II antibody, ICH-2 antibody, Mih1/TX antibody, TX antibody, Casp11 antibody, CASP13 antibody, CASP1 antibody, CASP4 antibody, Caspl antibody, ich-3 antibody, Casp4 antibody, caspase 4 antibody, caspase 4, apoptosis-related cysteine peptidase antibody, caspase-4 antibody, CASP4 antibody, Casp4 antibody, LOC100731032 antibody

Background: Caspase 4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue, and belongs to a family of cysteine proteases called caspases. The Caspase 4 gene is mapped to a P1 clone containing the ICE gene, which is located at chromosome 11q22.2-q22.3. It contains 8 coding exons.The function of caspase 4 is not fully known, but it is believed to be an inflammatory caspase, along with caspase 1, caspase 5(and the murine homolog caspase 11), with a role in the immune system.

Synonyms: Apoptosis related cysteine peptidase antibody|Apoptotic cysteine protease Mih1/TX antibody|CASP 4 antibody|CASP 4 antibody|CASP-4 antibody|CASP4 antibody|CASP4_HUMAN antibody|Caspase 4 apoptosis related cysteine peptidase antibody|Caspase-4 subunit 2 antibody|Caspase4 antibody|ICE(rel) II antibody|ICE(rel)-II antibody|ICE(rel)II antibody|ICEREL II antibody|ICERELII antibody|ICH 2 antibody|ICH 2 protease antibody|ICH2 antibody|Mih1/TX antibody|Mih1/TX antibody|Protease ICH-2 antibody|Protease TX antibody|TX antibody|TX protease antibody

UniProt: P49662

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity