DDT antibody (C-Term)

Catalog No. ABIN3043212

Product Details anti-DDT Antibody

Target: DDT (D-Dopachrome Tautomerase (DDT))

Binding Specificity: AA 99-118, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for D-dopachrome decarboxylase(DDT) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: RFFPLESWQI GKIGTVMTFL

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for D-dopachrome decarboxylase(DDT) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: D-dopachrome tautomerase
Protein Name: D-dopachrome decarboxylase

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DDT(99-118aa RFFPLESWQIGKIGTVMTFL), different from the related mouse and rat sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DDT

Target: DDT (D-Dopachrome Tautomerase (DDT))

Alternative Name: DDT (DDT Products)

Synonyms: ddt-b antibody, zgc:86714 antibody, wu:fb49f11 antibody, C78655 antibody, DDT antibody, ddt antibody, ddt-a antibody, DDCT antibody, D-dopachrome tautomerase L homeolog antibody, D-dopachrome tautomerase antibody, D-dopachrome tautomerase S homeolog antibody, ddt.L antibody, DDT antibody, ddt antibody, Ddt antibody, ddt.S antibody

Background: DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure.the genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10.

Synonyms: D dopachrome decarboxylase antibody|D dopachrome tautomerase antibody|D-dopachrome decarboxylase antibody|D-dopachrome tautomerase antibody|DDCT antibody|Ddt antibody|Phenylpyruvate tautomerase II antibody

UniProt: P30046