CA3 antibody (N-Term)

Catalog No. ABIN3043222

Product Details anti-CA3 Antibody

Target: CA3 (Carbonic Anhydrase III (CA3))

Binding Specificity: AA 2-20, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Carbonic anhydrase 3(CA3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: AKEWGYASHN GPDHWHELF

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Carbonic anhydrase 3(CA3) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: carbonic anhydrase III, muscle specific
Protein Name: Carbonic anhydrase 3

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CA3 (2-20aa AKEWGYASHNGPDHWHELF), different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CA3

Target: CA3 (Carbonic Anhydrase III (CA3))

Alternative Name: CA3 (CA3 Products)

Synonyms: CAIII antibody, Car3 antibody, BB219044 antibody, Ca3 antibody, Car-3 antibody, carbonic anhydrase 3 antibody, CA3 antibody, CAH3 antibody, Car3 antibody, Ca3 antibody

Background: Carbonic anhydrase III(CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family(at least six separate genes are known) that encode carbonic anhydrase isozymes. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. CA3 is found in high concentration in muscle. CA3 is used in conjunction with creatine kinase detection of the carrier state for Duchenne muscular dystrophy.

Synonyms: CA III antibody|CA-III antibody|Ca3 antibody|CAH3_HUMAN antibody|CAIII antibody|Car3 antibody|Carbonate dehydratase III antibody|Carbonic anhydrase 3 antibody|Carbonic anhydrase III antibody|Carbonic anhydrase III muscle specific antibody|EC 4.2.1.1 antibody

UniProt: P07451