SERPINH1 antibody (AA 247-418)

Catalog No. ABIN3043331

Product Details anti-SERPINH1 Antibody

Target: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Binding Specificity: AA 247-418

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SERPINH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Serpin H1(SERPINH1) detection. Tested with WB, IHC-P in Human,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Serpin H1(SERPINH1) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
Protein Name: Serpin H1

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Hsp47 recombinant protein (Position: D247-L418). Human Hsp47 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp47.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Hsp47 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for SERPINH1

Target: SERPINH1 (Serpin Family H Member 1 (SERPINH1))

Alternative Name: SERPINH1 (SERPINH1 Products)

Synonyms: AsTP3 antibody, CBP1 antibody, CBP2 antibody, HSP47 antibody, OI10 antibody, PPROM antibody, RA-A47 antibody, SERPINH2 antibody, gp46 antibody, SERPINH1 antibody, DKFZp468B197 antibody, cb216 antibody, hsp antibody, hsp47 antibody, id:ibd5060 antibody, wu:fc56b02 antibody, BERF-1 antibody, Cbp1 antibody, Cbp2 antibody, Hsp47 antibody, J6 antibody, Serpinh2 antibody, colligin antibody, astp3 antibody, cbp1 antibody, cbp2 antibody, pig14 antibody, pprom antibody, ra-a47 antibody, serpinh2 antibody, serpin family H member 1 antibody, serpin peptidase inhibitor, clade H (heat shock protein 47), member 1b antibody, serpin H1 antibody, selenoprotein K antibody, serine (or cysteine) peptidase inhibitor, clade H, member 1 antibody, serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) L homeolog antibody, SERPINH1 antibody, serpinh1b antibody, LOC100009353 antibody, Selenok antibody, Serpinh1 antibody, serpinh1.L antibody

Target Type: Viral Protein

Background: Heat shock protein 47, also known as SERPINH1 or HSP47, is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen, thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. It has been found that HSP47 monitors the integrity of the triple helix of type I procollagen at the ER/cis-Golgi boundary and, when absent, the rate of transit from the ER to the Golgi is increased and the helical structure is compromised.

Synonyms: 47 kDa heat shock protein antibody|47 kDa heat shock protein precursor antibody|Arsenic transactivated protein 3 antibody|Arsenic-transactivated protein 3 antibody|AsTP 3 antibody|AsTP3 antibody|CBP 1 antibody|CBP 2 antibody|CBP1 antibody|CBP2 antibody|Cell proliferation-inducing gene 14 protein antibody|Collagen binding protein 1 antibody|Collagen binding protein 2 antibody|Collagen binding protein antibody|Collagen-binding protein 2 antibody|Collagen-binding protein antibody|Colligen antibody|Colligin 1 antibody|Colligin 2 antibody|Colligin antibody|colligin-1 antibody|colligin-2 antibody|gp46 antibody|Heat shock protein 47 antibody|Heat-shock protein 47 antibody|HGNC 1547 antibody|Hsp 47 antibody|J6 antibody|OI10 antibody|PIG 14 antibody|PIG14 antibody|PPROM antibody|Proliferation inducing gene 14 antibody|Proliferation inducing gene 14 protein antibody|RA A47 antibody|RA-A47 antibody|Rheumatoid arthritis antigen A 47 antibody|rheumatoid arthritis antigen A-47 antibody|Rheumatoid arthritis related antigen RA A47 antibody|Rheumatoid arthritis-related antigen RA-A47 antibody|serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody|serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2) antibody|Serine or cysteine proteinase inhibitor clade H member 1 antibody|Serine or cysteine proteinase inhibitor clade H member 2 antibody|SERPH_HUMAN antibody|Serpin H1 antibody|Serpin peptidase inhibitor clade H member 1 antibody|serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody|Serpin peptidase inhibitor, clade H, member 1 antibody|SERPINH1 antibody|SERPINH2 antibody

Gene ID: 871

UniProt: P50454