CD34 antibody (AA 151-385)

Catalog No. ABIN3043391

Product Details anti-CD34 Antibody

Target: CD34

Binding Specificity: AA 151-385

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Hematopoietic progenitor cell antigen CD34(CD34) detection. Tested with WB, IHC-P, FCM in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Hematopoietic progenitor cell antigen CD34(CD34) detection. Tested with WB, IHC-P, FCM in Human,Mouse,Rat.
Gene Name: CD34 Molecule
Protein Name: Hematopoietic progenitor cell antigen CD34

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD34 recombinant protein (Position: T151-L385). Human CD34 shares 79% amino acid (aa) sequence identity with mouse CD34.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD34 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Flow Cytometry: Concentration:1-3 μg/1x106 cells, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-CD34 antibody (ABIN3043391)

Zhang, Chen, Wang, Yang, Li, Wang, Liu, Ye: "Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase." in: Oncotarget, Vol. 7, Issue 26, pp. 39302-39315, (2018) (PubMed).

Hu, Li, Zhang, Zheng, Wang, Zhang, Zhang, Gu, Ye, Guo, Yang, Wang: "Phosphoinositide 3-Kinase (PI3K) Subunit p110δ Is Essential for Trophoblast Cell Differentiation and Placental Development in Mouse." in: Scientific reports, Vol. 6, pp. 28201, (2018) (PubMed).

Qin, Ke, Zhou, Wang, Liang, Wang, Yang, Gao, Ye, Kumar, Wang: "Metastasis-Associated Protein 1 Deficiency Results in Compromised Pulmonary Alveolar Capillary Angiogenesis in Mice." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 3932-3941, (2018) (PubMed).

Chu, Zhang: "Inhibition of angiogenesis by leflunomide via targeting the soluble ephrin-A1/EphA2 system in bladder cancer." in: Scientific reports, Vol. 8, Issue 1, pp. 1539, (2018) (PubMed).

Zhang, Yuan, Wang, Shao, Liu, Firestone, Hong, Li, Xin, Li: "Experimental evidence of good efficacy and reduced toxicity with peptide-doxorubicin to treat gastric cancer." in: Oncotarget, Vol. 9, Issue 2, pp. 1957-1968, (2018) (PubMed).

Shi, Lv, Nie, Lu, Liu, Tian, Li, Zhang, Ren, Zhang, Kang: "Human nail stem cells are retained but hypofunctional during aging." in: Journal of molecular histology, Vol. 49, Issue 3, pp. 303-316, (2018) (PubMed).

Zhao, Feng, Sun, Qin, Zhang, Tan, Gao, Lau, Ma, Yang, Yu, Xu, Yi, Yi: "Cardiac-derived CTRP9 protects against myocardial ischemia/reperfusion injury via calreticulin-dependent inhibition of apoptosis." in: Cell death & disease, Vol. 9, Issue 7, pp. 723, (2018) (PubMed).

Chen, Zhou, Yao, Dai, Zhou, Wang, Zhang: "Dipalmitoylphosphatidic acid inhibits breast cancer growth by suppressing angiogenesis via inhibition of the CUX1/FGF1/HGF signalling pathway." in: Journal of cellular and molecular medicine, Vol. 22, Issue 10, pp. 4760-4770, (2018) (PubMed).

Wan, Wu, Huang, Ge, Liu: "Incomplete radiofrequency ablation accelerates proliferation and angiogenesis of residual lung carcinomas via HSP70/HIF-1α." in: Oncology reports, Vol. 36, Issue 2, pp. 659-68, (2017) (PubMed).

Gao, Chen, Gao, Wang, Sun, Yang: "CD47 deficiency in tumor stroma promotes tumor progression by enhancing angiogenesis." in: Oncotarget, Vol. 8, Issue 14, pp. 22406-22413, (2017) (PubMed).

Wan, Wu: "Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways." in: Journal of experimental & clinical cancer research : CR, Vol. 35, Issue 1, pp. 119, (2017) (PubMed).

Atala: "Re: Treatment of Diabetes Mellitus-Induced Erectile Dysfunction Using Endothelial Progenitor Cells Genetically Modified with Human Telomerase Reverse Transcriptase." in: The Journal of urology, Vol. 197, Issue 2, pp. 494, (2017) (PubMed).

Shi, Jiang, Wang, Cao, Liu, Xu, Wang, Sun, Niu: "Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device." in: Cell cycle (Georgetown, Tex.), Vol. 14, Issue 19, pp. 3058-65, (2016) (PubMed).

Wan, Wu, Zhang: "Local recurrence of small cell lung cancer following radiofrequency ablation is induced by HIF-1α expression in the transition zone." in: Oncology reports, Vol. 35, Issue 3, pp. 1297-308, (2016) (PubMed).

Zhang, Shang, Hao, Zheng, Li, Liang, Cui, Liu: "Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats." in: American journal of translational research, Vol. 7, Issue 11, pp. 2176-86, (2016) (PubMed).

Zhang, Wang, Dai, Cai, Zhan, Gang, Hu, Xia, Zhang: "Lentivirus-mediated PLCγ1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma." in: Oncotarget, Vol. 7, Issue 7, pp. 8043-54, (2016) (PubMed).

Jiang, Li, Wang, Xiao, Li, Liu, Liu: "Assessment of Vascularity in Hepatic Alveolar Echinococcosis: Comparison of Quantified Dual-Energy CT with Histopathologic Parameters." in: PLoS ONE, Vol. 11, Issue 2, pp. e0149440, (2016) (PubMed).

Miao, Guo, Li, Ma: "Radix Ilicis Pubescentis total flavonoids combined with mobilization of bone marrow stem cells to protect against cerebral ischemia/reperfusion injury." in: Neural regeneration research, Vol. 11, Issue 2, pp. 278-84, (2016) (PubMed).

Zhang, Song, Jia, Zhang: "Buyanghuanwu decoction promotes angiogenesis after cerebral ischemia/reperfusion injury: mechanisms of brain tissue repair." in: Neural regeneration research, Vol. 11, Issue 3, pp. 435-40, (2016) (PubMed).

Jiang, Han, Li, Yang, Liu: "Carboxymethyl chitosan represses tumor angiogenesis in vitro and in vivo." in: Carbohydrate polymers, Vol. 129, pp. 1-8, (2015) (PubMed).

Target Details for CD34

Target: CD34

Alternative Name: CD34 (CD34 Products)

Synonyms: AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody

Background: CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30 % of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.

Synonyms: CD34 antibody|Cd34 antibody|CD34 antigen antibody|CD34 Molecule antibody|CD34_HUMAN antibody|Cluster designation 34 antibody|Hematopoietic progenitor cell antigen CD34 antibody|HPCA1 antibody|Mucosialin antibody|OTTHUMP00000034733 antibody|OTTHUMP00000034734 antibody

Gene ID: 947

UniProt: P28906