SLC1A2 antibody (AA 461-574)

Catalog No. ABIN3043436

Product Details anti-SLC1A2 Antibody

Target: SLC1A2 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2))

Binding Specificity: AA 461-574

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC1A2 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Excitatory amino acid transporter 2(SLC1A2) detection. Tested with WB in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Excitatory amino acid transporter 2(SLC1A2) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: solute carrier family 1 (glial high affinity glutamate transporter), member 2
Protein Name: Excitatory amino acid transporter 2

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human EAAT2 recombinant protein (Position: T461-K574). Human EAAT2 shares 96% amino acid (aa) sequence identity with both mouse and rat EAAT2.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for EAAT2 is approximately 0.1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-SLC1A2 antibody (ABIN3043436)

Ding, Zhang, Liu, Zhang, Ma, Bruce, Zhang: "Tumor necrosis factor-? promotes the expression of excitatory amino-acid transporter 2 in astrocytes: Optimal concentration and incubation time." in: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1909-1913, (2014) (PubMed).

Target Details for SLC1A2

Target: SLC1A2 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 2 (SLC1A2))

Alternative Name: SLC1A2 (SLC1A2 Products)

Synonyms: CG3159 antibody, Dmel\\CG3159 antibody, EAAT antibody, dEAAT2 antibody, dEaat2 antibody, Eaat antibody, GB16377 antibody, EAAT2 antibody, SLC1A2 antibody, Eaat2 antibody, Glt antibody, Glt-1 antibody, GLT-1 antibody, 1700091C19Rik antibody, 2900019G14Rik antibody, AI159670 antibody, GLT1 antibody, MGLT1 antibody, eaat2 antibody, fj34b12 antibody, slc1a2 antibody, wu:fj34b12 antibody, zgc:65897 antibody, Excitatory amino acid transporter 2 antibody, excitatory amino acid transporter 2 antibody, solute carrier family 1 member 2 antibody, solute carrier family 1 (glial high affinity glutamate transporter), member 2 antibody, solute carrier family 1 (glial high affinity glutamate transporter), member 2b antibody, Eaat2 antibody, Eaat-2 antibody, SLC1A2 antibody, VDBG_06180 antibody, EUBELI_01747 antibody, Trebr_0139 antibody, Slc1a2 antibody, slc1a2b antibody

Background: SLC1A2 is also known as EAAT2 or GLT-1. This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified.

Synonyms: EAA2_HUMAN antibody|EAAT2 antibody|Excitatory amino acid transporter 2 antibody|Excitotoxic amino acid transporter 2 antibody|Glial high affinity glutamate transporter antibody|GLT 1 antibody|GLT1 antibody|Glutamate aspartate transporter II antibody|Glutamate transporter 1 antibody|Glutamate/aspartate transporter II antibody|Slc1a2 antibody|Sodium dependent glutamate aspartate transporter 2 antibody|Sodium-dependent glutamate/aspartate transporter 2 antibody|solute carrier family 1 (glial high affinity glutamate transporter), member 2 antibody|Solute carrier family 1 glial high affinity glutamate transporter member 2 antibody|Solute carrier family 1 member 2 antibody

Gene ID: 6506

UniProt: P43004

Pathways: Dicarboxylic Acid Transport