BMP2 antibody (C-Term)

Catalog No. ABIN3043489

Product Details anti-BMP2 Antibody

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Binding Specificity: AA 283-312, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Bone morphogenetic protein 2(BMP2) detection. Tested with WB, IHC-P, ELISA in Human,Rat.

Sequence: QAKHKQRKRL KSSCKRHPLY VDFSDVGWND

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Bone morphogenetic protein 2(BMP2) detection. Tested with WB, IHC-P, ELISA in Human,Rat.
Gene Name: bone morphogenetic protein 2
Protein Name: Bone morphogenetic protein 2

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human BMP-2 (283-312aa QAKHKQRKRLKSSCKRHPLYVDFSDVGWND), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Human

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-BMP2 antibody (ABIN3043489)

Jian, Fan, Hu, He, Li, Zheng, Ren, Li: "Effects of rhBMP-2 gene transfection to periodontal ligament cells on osteogenesis." in: Bioscience reports, Vol. 37, Issue 3, (2018) (PubMed).

Fernández, Córdoba: "Hyaluronic acid-induced capacitation involves protein kinase C and tyrosine kinase activity modulation with a lower oxidative metabolism in cryopreserved bull sperm." in: Theriogenology, Vol. 122, pp. 68-73, (2018) (PubMed).

Xu, Sun, Zhang, Xu, Du, Zhang, Wang: "Diabetes mellitus affects the biomechanical function of the callus and the expression of TGF-beta1 and BMP2 in an early stage of fracture healing." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, Vol. 49, Issue 1, pp. e4736, (2016) (PubMed).

Liu, Chen, Zeng, Cui, Ning, Wang, Belguise, Wang, Qian, Lu, Yi: "Bone morphogenic protein-2 regulates the myogenic differentiation of PMVECs in CBDL rat serum-induced pulmonary microvascular remodeling." in: Experimental cell research, Vol. 336, Issue 1, pp. 109-18, (2015) (PubMed).

Wang, Xue, Zhao, Liu, Ma, Ma: "Form-deprivation myopia induces decreased expression of bone morphogenetic protein-2, 5 in guinea pig sclera." in: International journal of ophthalmology, Vol. 8, Issue 1, pp. 39-45, (2015) (PubMed).

Chai, Guo, Wang, Gao, Liu, Fu, Guan, Tan, Yang: "In vitro and in vivo evaluations on osteogenesis and biodegradability of a β-tricalcium phosphate coated magnesium alloy." in: Journal of biomedical materials research. Part A, Vol. 100, Issue 2, pp. 293-304, (2014) (PubMed).

Guo, Zeng, Yan, Shen, Liu, Li, Zhang, Wu, Guan, Huang: "Proliferative effect and osteoinductive potential of extracellular matrix coated on cell culture plates." in: SpringerPlus, Vol. 2, Issue 1, pp. 303, (2014) (PubMed).

Sun, Sun, Tian, Xu, Zhou, Xi, Yan, Wang: "A comparison of osteocyte bioactivity in fine particulate bone powder grafts vs larger bone grafts in a rat bone repair model." in: Acta histochemica, Vol. 116, Issue 6, pp. 1015-21, (2014) (PubMed).

Zhong, Liu, Dai, Zhou, Fu: "Sodium thiosulfate protects human aortic smooth muscle cells from osteoblastic transdifferentiation via high-level phosphate." in: The Kaohsiung journal of medical sciences, Vol. 29, Issue 11, pp. 587-93, (2013) (PubMed).

Qiao, Zhang, Jin, Miao, Shi, Liu, Yuan, Liu, Li, Zheng, Zhang, Li, Yang, Sun: "Using poly(lactic-co-glycolic acid) microspheres to encapsulate plasmid of bone morphogenetic protein 2/polyethylenimine nanoparticles to promote bone formation in vitro and in vivo." in: International journal of nanomedicine, Vol. 8, pp. 2985-95, (2013) (PubMed).

Li, Chen, Wu, Jiang, Ge, Gao, Zhang, Wu: "Enhancement of the osseointegration of a polyethylene terephthalate artificial ligament graft in a bone tunnel using 58S bioglass." in: International orthopaedics, Vol. 36, Issue 1, pp. 191-7, (2012) (PubMed).

Wang, Liu, Dang, Ma, Zhang, Wang: "The effect of core decompression on local expression of BMP-2, PPAR-γ and bone regeneration in the steroid-induced femoral head osteonecrosis." in: BMC musculoskeletal disorders, Vol. 13, pp. 142, (2012) (PubMed).

Chen, Kong, Wan, Xiao, Li, Wang, Lin, Wang: "Effects of huogu I formula (I) on correlated factors of bone regeneration in chickens with steroid-induced necrosis of femoral head." in: Chinese journal of integrative medicine, Vol. 18, Issue 5, pp. 378-84, (2012) (PubMed).

Liao, Chen, Li, Ge, Duan, Zou, Ge: "Transfer of bone-marrow-derived mesenchymal stem cells influences vascular remodeling and calcification after balloon injury in hyperlipidemic rats." in: Journal of biomedicine & biotechnology, Vol. 2012, pp. 165296, (2012) (PubMed).

Yao, Wang, Wang, Wang, Zhang, Liu: "Synergistic enhancement of new bone formation by recombinant human bone morphogenetic protein-2 and osteoprotegerin in trans-sutural distraction osteogenesis: a pilot study in dogs." in: Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, Vol. 69, Issue 11, pp. e446-55, (2011) (PubMed).

Han, Li, Guan: "Ectopic osteogenesis of hBMP-2 gene-transduced human bone mesenchymal stem cells/BCB." in: Connective tissue research, Vol. 51, Issue 4, pp. 274-81, (2010) (PubMed).

Liu, Zhong, Liang, Fu, Luo, Zhou, Gou, Huang: "Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway." in: Journal of Zhejiang University. Science. B, Vol. 11, Issue 12, pp. 905-11, (2010) (PubMed).

Ma, Ma, Guo, Zhang: "Expression of bone morphogenetic protein-2 and its receptors in epithelial ovarian cancer and their influence on the prognosis of ovarian cancer patients." in: Journal of experimental & clinical cancer research : CR, Vol. 29, pp. 85, (2010) (PubMed).

Tian, Sun, Zhang, Gao, Fu, Yang: "Construction and expression of a bicistronic vector containing human bone morphogenetic protein 2 and vascular endothelial growth factor-165 genes in vitro." in: Chinese medical journal, Vol. 122, Issue 4, pp. 471-3, (2009) (PubMed).

Hu, Cui, Yang, Wang, Hu, Li, Zeng: "Bone morphogenetic protein-2: a potential regulator in scleral remodeling." in: Molecular vision, Vol. 14, pp. 2373-80, (2008) (PubMed).

Target Details for BMP2

Target: BMP2 (Bone Morphogenetic Protein 2 (BMP2))

Alternative Name: BMP2 (BMP2 Products)

Synonyms: BDA2 antibody, BMP2A antibody, AI467020 antibody, Bmp2a antibody, BMP-2 antibody, xBMP-2 antibody, xbmp2 antibody, BMP2 antibody, bmp2a antibody, bmp2 antibody, wu:fc59d09 antibody, bone morphogenetic protein 2 antibody, bone morphogenetic protein 2 L homeolog antibody, Bone morphogenetic protein 2 antibody, bone morphogenetic protein 2a antibody, BMP2 antibody, Bmp2 antibody, bmp2.L antibody, bmp2 antibody, bmp2a antibody

Background: BMP2 is also known as Bone morphogenetic protein 2 or BMP2A. It is mapped to 20p12. The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. BMP-2, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. It is involved in the hedgehog pathway, TGF beta signaling pathway, and in cytokine-cytokine receptor interaction. Also, it is involved in cardiac cell differentiation and epithelial to mesenchymal transition. In addition, BMP2A has been suggested as a reasonable candidate for the human condition fibrodysplasia (myositis) ossificans progressiva, on the basis of observations in a Drosophila model.

Synonyms: BDA2 antibody|BMP-2 antibody|BMP-2A antibody|Bmp2 antibody|BMP2_HUMAN antibody|BMP2A antibody|Bone morphogenetic protein 2 antibody| Bone morphogenetic protein 2A antibody

Gene ID: 650

UniProt: P12643

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation