DLD antibody (AA 300-509)

Catalog No. ABIN3043561

Product Details anti-DLD Antibody

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Binding Specificity: AA 300-509

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Dihydrolipoyl dehydrogenase, mitochondrial(DLD) detection. Tested with WB, IHC-P in Human,Mouse, Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Dihydrolipoyl dehydrogenase, mitochondrial(DLD) detection. Tested with WB, IHC-P in Human,Mouse, Rat.
Gene Name: dihydrolipoamide dehydrogenase
Protein Name: Dihydrolipoyl dehydrogenase, mitochondrial

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DLD recombinant protein (Position: K300-F509). Human DLD shares 96.2% and 95.7% amino acid (aa) sequence identity with mouse and rat DLD, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for DLD

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Alternative Name: DLD (DLD Products)

Synonyms: DLDD antibody, DLDH antibody, E3 antibody, GCSL antibody, LAD antibody, PHE3 antibody, AI315664 antibody, AI746344 antibody, wu:fb24b05 antibody, DLD antibody, DDBDRAFT_0183800 antibody, DDBDRAFT_0216232 antibody, DDB_0183800 antibody, DDB_0216232 antibody, sc:d0402 antibody, dihydrolipoamide dehydrogenase antibody, dihydrolipoyl dehydrogenase antibody, deltaD antibody, DLD antibody, Dld antibody, dldh antibody, AT4G16155 antibody, CND05840 antibody, bfmBC antibody, GCSL antibody, LACBIDRAFT_182385 antibody, UREG_06178 antibody, lpd antibody, TAGG_RS02070 antibody, Arnit_2606 antibody, Mesil_1945 antibody, Trad_2118 antibody, Acear_0640 antibody, Fbal_0372 antibody, Ilyop_1890 antibody, Ftrac_1733 antibody, Ocepr_1753 antibody, Intca_2017 antibody, Deima_0504 antibody, dld antibody

Background: DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex (BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.

Synonyms: Diaphorase antibody|Dihydrolipoamide dehydrogenase antibody|Dihydrolipoyl dehydrogenase antibody|Dihydrolipoyl dehydrogenase mitochondrial antibody|dld antibody|DLDH antibody|DLDH_HUMAN antibody|E3 antibody|E3 component of pyruvate dehydrogenase antibody|E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibody|GCSL antibody|Glycine cleavage system L protein antibody|Glycine cleavage system protein L antibody|LAD antibody|lipoamide dehydrogenase antibody|Lipoamide reductase antibody|Lipoyl dehydrogenase antibody|mitochondrial antibody|OTTHUMP00000206744 antibody| OTTHUMP00000206746 antibody|OTTHUMP00000206748 antibody|OTTHUMP00000206749 antibody|PHE 3 antibody|PHE3 antibody

Gene ID: 1738

UniProt: P09622

Pathways: Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis