RCAS1 antibody (AA 31-213)

Catalog No. ABIN3043569

Product Details anti-RCAS1 Antibody

Target: RCAS1 (EBAG9) (Estrogen Receptor Binding Site Associated, Antigen, 9 (EBAG9))

Binding Specificity: AA 31-213

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RCAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Receptor-binding cancer antigen expressed on SiSo cells(EBAG9) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Receptor-binding cancer antigen expressed on SiSo cells(EBAG9) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: estrogen receptor binding site associated, antigen, 9
Protein Name: Receptor-binding cancer antigen expressed on SiSo cells

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human EBAG9 recombinant protein (Position: R31-S213). Human EBAG9 shares 97.8% and 94.5% amino acid (aa) sequence identity with mouse and rat EBAG9, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for RCAS1

Target: RCAS1 (EBAG9) (Estrogen Receptor Binding Site Associated, Antigen, 9 (EBAG9))

Alternative Name: EBAG9 (EBAG9 Products)

Synonyms: EB9 antibody, PDAF antibody, MGC56608 antibody, zgc:56608 antibody, MGC84425 antibody, EBAG9 antibody, AI835379 antibody, Rcas1 antibody, RCAS1 antibody, eb9 antibody, pdaf antibody, rcas1 antibody, estrogen receptor binding site associated, antigen, 9 antibody, estrogen receptor binding site associated, antigen, 9 L homeolog antibody, estrogen receptor-binding fragment-associated gene 9 antibody, EBAG9 antibody, ebag9 antibody, ebag9.L antibody, Ebag9 antibody

Background: Receptor-binding cancer antigen expressed on SiSo cells is a protein that in humans is encoded by the EBAG9 gene. This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. And the encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10.

Synonyms: BAG9 antibody|Cancer associated surface antigen antibody|Cancer associated surface antigen RCAS1 antibody|Cancer-associated surface antigen RCAS1 antibody|EB9 antibody|EBAG 9 antibody|EBAG9 antibody|Estrogen receptor binding fragment associated gene 9 protein antibody|Estrogen receptor binding site associated antigen 9 antibody|estrogen receptor binding site associated, antigen, 9 antibody|Estrogen receptor-binding fragment-associated gene 9 protein antibody|PDAF antibody|RCAS 1 antibody|RCAS1 antibody|RCAS1_HUMAN antibody| Receptor binding cancer antigen expressed on SiSo cells antibody|Receptor-binding cancer antigen expressed on SiSo cells antibody

Gene ID: 9166

UniProt: O00559