PBK antibody (AA 71-322)

Catalog No. ABIN3043592

Product Details anti-PBK Antibody

Target: PBK (PDZ Binding Kinase (PBK))

Binding Specificity: AA 71-322

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PBK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Lymphokine-activated killer T-cell-originated protein kinase(PBK) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Lymphokine-activated killer T-cell-originated protein kinase(PBK) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: PDZ binding kinase
Protein Name: Lymphokine-activated killer T-cell-originated protein kinase

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PBK recombinant protein (Position: N71-V322). Human PBK shares 89% amino acid (aa) sequence identity with mouse PBK.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for PBK is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for PBK

Target: PBK (PDZ Binding Kinase (PBK))

Alternative Name: PBK (PBK Products)

Synonyms: PBK antibody, topk antibody, CT84 antibody, Nori-3 antibody, SPK antibody, TOPK antibody, 2810434B10Rik antibody, AW538537 antibody, D14Ertd732e antibody, zgc:92050 antibody, PDZ binding kinase antibody, PDZ binding kinase L homeolog antibody, PBK antibody, pbk antibody, Pbk antibody, pbk.L antibody

Background: Lymphokine-activated killer T-cell-originated protein kinase, also known as TOPK or PBK, is an enzyme that in humans is encoded by the PBK gene. The protein encoded by this gene is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. It is mapped to 8p21.1. PBK can mediate cell growth through histone H3 modification. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis.

Synonyms: Cancer/testis antigen 84 antibody|CT84 antibody|Epididymis luminal protein 164 antibody|FLJ14385 antibody|HEL164 antibody|Lymphokine activated killer T cell originated protein kinase antibody|Lymphokine-activated killer T-cell-originated protein kinase antibody|MAPKK like protein kinase antibody|MAPKK-like protein kinase antibody|Nori 3 antibody|Nori-3 antibody|Nori3 antibody|PBK antibody|PDZ binding kinase antibody|PDZ-binding kinase antibody|Serine/threonine protein kinase antibody|Spermatogenesis related protein kinase antibody|Spermatogenesis-related protein kinase antibody|SPK antibody|T LAK cell originated protein kinase antibody|T-LAK cell-originated protein kinase antibody|TOPK antibody|TOPK_HUMAN antibody

Gene ID: 55872