MAD2L1 antibody (AA 2-205)

Catalog No. ABIN3043608

Product Details anti-MAD2L1 Antibody

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Binding Specificity: AA 2-205

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAD2L1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Mitotic spindle assembly checkpoint protein MAD2A(MAD2L1) detection. Tested with WB in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Mitotic spindle assembly checkpoint protein MAD2A(MAD2L1) detection. Tested with WB in Human.
Gene Name: MAD2 mitotic arrest deficient-like 1 (yeast)
Protein Name: Mitotic spindle assembly checkpoint protein MAD2A

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Mad2L1 recombinant protein (Position: A2-D205). Human Mad2L1 shares 94% amino acid (aa) sequence identity with mouse Mad2L1.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Mad2L1 is approximately 0.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for MAD2L1

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Alternative Name: MAD2L1 (MAD2L1 Products)

Synonyms: HSMAD2 antibody, MAD2 antibody, MXD2 antibody, MXI antibody, bHLHc11 antibody, AA673185 antibody, cb408 antibody, zgc:112133 antibody, mad2 antibody, XMad2 antibody, MAD2L1 antibody, mitotic arrest deficient 2 like 1 antibody, MAX interactor 1, dimerization protein antibody, MAD2 mitotic arrest deficient-like 1 antibody, MAD2 mitotic arrest deficient-like 1 (yeast) antibody, mitotic arrest deficient 2 like 1 S homeolog antibody, MAD2L1 antibody, MXI1 antibody, Mad2l1 antibody, mad2l1 antibody, mad2l1.S antibody

Background: Mitotic spindle assembly checkpoint protein MAD2A is a protein that in humans is encoded by the MAD2L1 gene. This gene belongs to the MAD2 family. It is mapped to 4q27. MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. It is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment.

Synonyms: Homolog of MAD2 S cerevisiae antibody|HsMAD 2 antibody|HsMAD2 antibody|Human mitotic feedback control protein Madp2 antibody|MAD 2 antibody|MAD 2A antibody|MAD2 antibody|MAD2 like 1 antibody|MAD2 like protein 1 antibody|MAD2 mitotic arrest deficient like 1 antibody|MAD2-like protein 1 antibody|MAD2A antibody|Mad2l1 antibody|MD2L1_HUMAN antibody|Mitotic arrest deficient 2-like protein 1 antibody|Mitotic arrest deficient like 1 antibody|Mitotic arrest deficient yeast homolog antibody|Mitotic arrest deficient yeast homolog like 1 antibody|Mitotic spindle assembly checkpoint protein antibody|Mitotic spindle assembly checkpoint protein MAD2A antibody

Gene ID: 4085

UniProt: Q13257