ALOX15 antibody (AA 2-337)

Catalog No. ABIN3043726

Product Details anti-ALOX15 Antibody

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Binding Specificity: AA 2-337

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX15 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Arachidonate 15-lipoxygenase(ALOX15) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Arachidonate 15-lipoxygenase(ALOX15) detection. Tested with WB, IHC-P in Human.
Gene Name: arachidonate 15-lipoxygenase
Protein Name: Arachidonate 15-lipoxygenase

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ALOX15 recombinant protein (Position: G2-P337). Human ALOX15 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat ALOX15, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for ALOX15 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for ALOX15

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Alternative Name: ALOX15 (ALOX15 Products)

Synonyms: 15-LOX-1 antibody, 15LOX-1 antibody, ALOX15 antibody, 12-LO antibody, Alox12l antibody, L-12LO antibody, 12-LOX antibody, Alox12 antibody, ALOX12 antibody, 15-LOX antibody, Alox12e antibody, arachidonate 15-lipoxygenase antibody, Arachidonate 15-lipoxygenase antibody, ALOX15 antibody, Alox15 antibody, Nmul_A0532 antibody, Sden_1680 antibody, Swoo_2919 antibody, PCC8801_3106 antibody, Cyan8802_3014 antibody, Nwat_1315 antibody

Background: Arachidonate 15-lipoxygenase, also known as 15 LOX or LOG15, is an enzyme that in humans is encoded by the ALOX15 gene. This gene is mapped to 17p13.2. It is found that ALOX15 is a mutator gene. ALOX15 gene product is implicated in antiinflammation, membrane remodeling, and cancer development/metastasis. Loss of the TP53 gene, or gain-of-function activities resulting from the expression of its mutant forms regulates ALOX15 promoter activity in human and in mouse, albeit in directionally opposite manners.

Synonyms: 15 lipoxygenase 1 antibody|15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody|15 LOX antibody|15 LOX 1 antibody|15-LOX antibody|15LOX 1 antibody|ALOX15 antibody|Arachidonate 15 lipoxygenase antibody|Arachidonate 15-lipoxygenase antibody|Arachidonate omega 6 lipoxygenase antibody|Arachidonate omega-6 lipoxygenase antibody|LOG15 antibody|LOX15_HUMAN antibody

Gene ID: 246

UniProt: P16050

Pathways: Regulation of Actin Filament Polymerization