ABCC4 antibody (AA 1-370)

Catalog No. ABIN3043774

Product Details anti-ABCC4 Antibody

Target: ABCC4 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4))

Binding Specificity: AA 1-370

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Multidrug resistance-associated protein 4(ABCC4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Multidrug resistance-associated protein 4(ABCC4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 4
Protein Name: Multidrug resistance-associated protein 4

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MRP4 recombinant protein (Position: M1-P370).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for MRP4 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for ABCC4

Target: ABCC4 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4))

Alternative Name: ABCC4 (ABCC4 Products)

Synonyms: EST170205 antibody, MOAT-B antibody, MOATB antibody, MRP4 antibody, D630049P08Rik antibody, Mrp4 antibody, ATP binding cassette subfamily C member 4 antibody, multidrug resistance-associated protein 4 antibody, ATP-binding cassette, sub-family C (CFTR/MRP), member 4 antibody, ABCC4 antibody, LOC515333 antibody, LOC616707 antibody, LOC695407 antibody, abcc4 antibody, Abcc4 antibody

Background: ABCC4(Atp-binding cassette, subfamily c, member 4) also known as MRP4 or MOATB, is a protein that in humans is encoded by the ABCC4 gene. It belongs to a large family of transmembrane proteins involved in active transport of substrates out of cells. This gene is mapped to chromosome 13q32. ABCC4 acts as an independent regulator of intracellular cyclic nucleotide levels and as a mediator of cAMP-dependent signal transduction to the nucleus. The antiproliferative effect of ABCC4 inhibition was related to cAMP-dependent PKA activation and CREB phosphorylation. Pharmacologic inhibition of ABCC4 activity or knockdown of ABCC4 via RNA interference resulted in reduced migration of DCs from human skin explants and of in vitro-generated Langerhans cells. It has been found that ABCC4 contributes to migration of DCs toward draining lymph nodes and therefore has a role in the initiation of an immune response.

Synonyms: ABCC 4 antibody|ABCC4 antibody|ATP binding cassette sub family C (CFTR/MRP) member 4 antibody|ATP binding cassette sub family C member 4 antibody|ATP-binding cassette sub-family C member 4 antibody|bA464I2.1 (ATP binding cassette, sub-family C (CFTR/MRP) member 4) antibody|bA464I2.1 antibody|Canalicular multispecific organic anion transporter ABC superfamily antibody|canalicular multispecific organic anion transporter antibodyEST170205 antibody|MOAT B antibody|MOAT-B antibody|MOATB antibody|MRP 4 antibody|MRP/cMOAT related ABC transporter antibody|MRP/cMOAT-related ABC transporter antibody|MRP4_HUMAN antibody|Multi specific organic anion transporter B antibody|Multi-specific organic anion transporter B antibody|Multidrug resistance associated protein 4 antibody|Multidrug resistance-associated protein 4 antibody|OTTHUMP00000018560 antibody

Gene ID: 10257

UniProt: O15439