APP antibody (C-Term)

Catalog No. ABIN3043787

Product Details anti-APP Antibody

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Binding Specificity: AA 672-713, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Amyloid beta A4 protein(APP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: DAEFRHDSGY EVHHQKLVFF AEDVGSNKGA IIGLMVGGVV IA

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Amyloid beta A4 protein(APP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: amyloid beta (A4) precursor protein
Protein Name: Amyloid beta A4 protein

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human APP(672-713aa DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA), different from the related mouse and rat sequences by three amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for APP is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-APP antibody (ABIN3043787)

Sun, Zhou, Yi, Jiang, Yuan: "Lead-induced morphological changes and amyloid precursor protein accumulation in adult rat hippocampus." in: Biotechnic & histochemistry : official publication of the Biological Stain Commission, Vol. 89, Issue 7, pp. 513-7, (2014) (PubMed).

Qin, Yao, Huang: "Effects of compound danshen tablets on spatial cognition and expression of brain beta-amyloid precursor protein in a rat model of Alzheimer's disease." in: Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan / sponsored by All-China Association of Traditional Chinese Medicine, Academy of Traditional Chinese Medicine, Vol. 32, Issue 1, pp. 63-6, (2012) (PubMed).

Li, Li, Huang, Kan, Wang, Wu, Yin, Yao: "Dexamethasone and A???-?? accelerate learning and memory impairments due to elevate amyloid precursor protein expression and neuronal apoptosis in 12-month male rats." in: Behavioural brain research, Vol. 227, Issue 1, pp. 142-9, (2011) (PubMed).

Nie, Luo, Huang, Gong, Wu, Shi: "Icariin inhibits beta-amyloid peptide segment 25-35 induced expression of beta-secretase in rat hippocampus." in: European journal of pharmacology, Vol. 626, Issue 2-3, pp. 213-8, (2009) (PubMed).

Target Details for APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Alternative Name: APP (APP Products)

Synonyms: AAA antibody, ABETA antibody, ABPP antibody, AD1 antibody, APPI antibody, CTFgamma antibody, CVAP antibody, PN-II antibody, PN2 antibody, aaa antibody, abeta antibody, abpp antibody, ad1 antibody, appi antibody, ctfgamma antibody, cvap antibody, pn2 antibody, APP antibody, APP-like antibody, APPL antibody, Abeta antibody, BcDNA:GH04413 antibody, CG7727 antibody, Dmel\\CG7727 antibody, EG:65F1.5 antibody, appl antibody, Abpp antibody, Adap antibody, Ag antibody, Cvap antibody, E030013M08Rik antibody, betaApp antibody, app antibody, wu:fj34d10 antibody, wu:fk65e12 antibody, zgc:85740 antibody, amyloid beta precursor protein antibody, amyloid beta (A4) precursor protein antibody, beta amyloid protein precursor-like antibody, amyloid beta (A4) precursor protein a antibody, amyloid beta precursor protein L homeolog antibody, APP antibody, app antibody, Appl antibody, App antibody, appa antibody, app.L antibody

Background: Beta Amyloid, also called Abeta or Abeta, denotes peptides of 36-43 amino acidsthat are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. It is mapped to 19q13.12. Several potential activities have been discovered for beta Amyloid, including activation of kinase enzymes, functioning as atranscription factor, and anti-microbial activity (potentially associated with beta Amyloid's pro-inflammatoryactivity). Moreover, monomeric beta Amyloid is indicated to protect neurons by quenching metal-inducible oxygen radical generation and thereby inhibiting neurotoxicity.

Synonyms: A4 antibody|A4 antibody|A4_HUMAN antibody|AAA antibody|AAA antibody|ABETA antibody|ABETA antibody|ABPP antibody|ABPP antibody|AD1 antibody|AICD-50 antibody|AICD-57 antibody|AICD-59 antibody|AID(50) antibody|AID(57) antibody|AID(59) antibody|Alzheimer disease amyloid protein antibody|Alzheimers Disease Amyloid Protein antibody|Amyloid B antibody|amyloid beta (A4) precursor protein antibody|amyloid beta A4 protein antibody|Amyloid Beta A4 Protein Precursor antibody|Amyloid Beta antibody|Amyloid intracellular domain 50 antibody|Amyloid intracellular domain 57 antibody|Amyloid intracellular domain 59 antibody|Amyloid of Aging and Alzheimer Disease antibody|APP antibody|APPI antibody|APPI antibody|B Amyloid antibody|Beta APP antibody|beta-amyloid peptide antibody|Beta-APP40 antibody|Beta-APP42 antibody|C31 antibody|Cerebral Vascular Amyloid Peptide antibody|CTFgamma antibody|CVAP antibody|CVAP antibody|Gamma-CTF(50)antibody|Gamma-CTF(57) antibody|Gamma-CTF(59) antibody|peptidase nexin-II antibody|PN II antibody|PN-II antibody|PN2 antibody|PreA4 antibody|PreA4 antibody|Protease nexin II antibody|Protease nexin-II antibody|S-APP-alpha antibody|S-APP-beta antibody

Gene ID: 351

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour