CENPA antibody (AA 1-140)

Catalog No. ABIN3043810

Product Details anti-CENPA Antibody

Target: CENPA (Centromere Protein A (CENPA))

Binding Specificity: AA 1-140

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPA antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Histone H3-like centromeric protein A(CENPA) detection. Tested with WB in Human,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Histone H3-like centromeric protein A(CENPA) detection. Tested with WB in Human,Rat.
Gene Name: centromere protein A
Protein Name: Histone H3-like centromeric protein A

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CENPA recombinant protein (Position: M1-G140). Human CENPA shares 71.6% amino acid (aa) sequence identity with mouse CENPA.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for CENPA

Target: CENPA (Centromere Protein A (CENPA))

Alternative Name: CENPA (CENPA Products)

Synonyms: CENP-A antibody, CenH3 antibody, Cenp-A antibody, cenpx antibody, cenp-a antibody, sim2 antibody, Cenp-a antibody, RGD1563607 antibody, CENPA antibody, centromere protein A antibody, centromere protein-A antibody, centromeric histone-3 like protein antibody, centromere-specific histone H3 CENP-A antibody, cenp-A antibody, centromere protein A L homeolog antibody, histone H3-like centromeric protein A antibody, CENPA antibody, Cenpa antibody, CENP-A antibody, cenpa antibody, cenp-A antibody, cnp1 antibody, HAN_3g422 antibody, Cenp-a antibody, cenpa.L antibody, CMU_016480 antibody

Background: Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. And CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. In higher eukaryotes, the recruitment of CENP-A nucleosomes to existing centromeres is an epigenetic process, independent of the underlying DNA sequence. In S.pombe, de novo recruitment of the CENP-A to the centromere is believed to be controlled by "centromeric" heterochromatin surrounding the centromere, and by an RNAi mechanism. The RNAi is cut to form siRNA, this complexes with the protein Chp1, which then binds the centromeric heterochromatin. This helps recruit other proteins, ultimately resulting in a protein complex that forms cohesin between two sister chromatids at the centromeric heterochromatin. This cohesin is believed to be essential in replacing the centromere H3 with CENP-A. CENP-A is one of the epigenetic changes that is believed to distinguish centromeric DNA from other DNA. Once the CENP-A has been added, the centromere becomes self-propagating, and the surrounding heterochromatin/RNAi mechanism is no longer necessary.

Synonyms: CENP A antibody|CENP-A antibody|cenpa antibody|CENPA_HUMAN antibody|Centromere autoantigen A antibody|Centromere protein A 17 kDa antibody|Centromere protein A antibody|Histone H3 like centromeric protein A antibody|Histone H3-like centromeric protein A antibody

Gene ID: 1058

UniProt: P49450

Pathways: Chromatin Binding, Maintenance of Protein Location