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CYP2E1 antibody (AA 1-310)

CYP2E1 Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3043821
  • Target See all CYP2E1 Antibodies
    CYP2E1 (Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1))
    Binding Specificity
    • 15
    • 11
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-310
    Reactivity
    • 70
    • 50
    • 36
    • 15
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    Human, Mouse, Rat
    Host
    • 77
    • 7
    • 3
    Rabbit
    Clonality
    • 74
    • 13
    Polyclonal
    Conjugate
    • 44
    • 12
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CYP2E1 antibody is un-conjugated
    Application
    • 64
    • 27
    • 19
    • 14
    • 13
    • 9
    • 8
    • 7
    • 6
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purpose
    Rabbit IgG polyclonal antibody for Cytochrome P450 2E1(CYP2E1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Cytochrome P450 2E1(CYP2E1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Gene Name: cytochrome P450, family 2, subfamily E, polypeptide 1
    Protein Name: Cytochrome P450 2E1
    Purification
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human CYP2E1 recombinant protein (Position: M1-Y310). Human CYP2E1 shares 73% and 74% amino acid (aa) sequences identity with mouse and rat CYP2E1, respectively.
    Isotype
    IgG
    Top Product
    Discover our top product CYP2E1 Primary Antibody
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for CYP2E1 is approximately 0.2 ng/lane under reducing conditions.
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Fan, Jiang, Wang, Tan, Zeng, Wang, Chen, Qu, Gonzalez, Huang, Bi: "Wuzhi tablet (Schisandra Sphenanthera extract) protects against acetaminophen-induced hepatotoxicity by inhibition of CYP-mediated bioactivation and regulation of NRF2-ARE and p53/p21 pathways." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 42, Issue 12, pp. 1982-90, (2014) (PubMed).

    Jiang, Fan, Wang, Chen, Zeng, Tan, Gonzalez, Huang, Bi: "Schisandrol B protects against acetaminophen-induced hepatotoxicity by inhibition of CYP-mediated bioactivation and regulation of liver regeneration." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 143, Issue 1, pp. 107-15, (2014) (PubMed).

    Chen, Sun, Han, Peng, Li, Liu, Lv, Liu, Zhou, Sun: "Protective effect of tea polyphenols against paracetamol-induced hepatotoxicity in mice is significantly correlated with cytochrome P450 suppression." in: World journal of gastroenterology, Vol. 15, Issue 15, pp. 1829-35, (2009) (PubMed).

  • Target
    CYP2E1 (Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1))
    Alternative Name
    CYP2E1 (CYP2E1 Products)
    Synonyms
    CYP2E1 antibody, CYP2E antibody, CYPIIE1 antibody, CPE1 antibody, P450-J antibody, P450C2E antibody, Cyp2e antibody, cytochrome P450, family 2, subfamily E, polypeptide 1 antibody, cytochrome P450 2E1 antibody, cytochrome P450 2E1-like antibody, cytochrome P450 family 2 subfamily E member 1 antibody, cytochrome P450, family 2, subfamily e, polypeptide 1 antibody, CYP2E1 antibody, PTRG_07411 antibody, LOC100342572 antibody, LOC100727914 antibody, LOC101843716 antibody, Cyp2e1 antibody
    Background
    Cytochrome P450 2E1 (abbreviated CYP2E1), a member of the cytochrome P450 mixed-function oxidase system, is involved in the metabolism of xenobiotics in the body. In humans, the CYP2E1 enzyme is encoded by the CYP2E1 gene. It is mapped to 10q26.3. While it is involved in the oxidative metabolism of a small range of substrates (mostly small polar molecules), there are many important drug interactions mediated by CYP2E1. Most drugs undergo deactivation by CYP2E1, either directly or by facilitated excretion from the body. Also, many substances are bioactivated by CYP2E1 to form their active compounds. In addition, CYP2E1 is an important enzyme for the conversion of ethanol to acetaldehyde and to acetate in humans. In the conversion sequence of acetyl-CoA to glucose, CYP2E1 transforms acetone via acetol into propylene glycol and methylglyoxal, the precursors of pyruvate, acetate and lactate.

    Synonyms: 4-nitrophenol 2-hydroxylase antibody|CP2E1_HUMAN antibody|CPE 1 antibody|CPE1 antibody|CYP2E antibody|CYP2E1 antibody|CYPIIE1 antibody|Cytochrome P450 2E1 antibody|cytochrome P450 family 2 subfamily E polypeptide 1 antibody|Cytochrome P450 isozyme 3A antibody|Cytochrome P450 LM3A antibody|Cytochrome P450 subfamily IIE (ethanol inducible) polypeptide 1 antibody|Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody|cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 antibody|Cytochrome P450, subfamily IIE antibody|Cytochrome P450-ALC antibody|Cytochrome P450-J antibody|Cytochrome P450RLM6 antibody|EC 1.14.13.- antibody|EC 1.14.13.n7 antibody|Ethanol inducible p450 antibody|Ethanol-inducible P450 antibody|flavoprotein-linked monooxygenase antibody|MGC133529 antibody|microsomal monooxygenase antibody|OTTHUMP00000020813 antibody|OTTHUMP00000046571 antibody|P450 ALC antibody|P450 J antibody|P450-J antibody|P450C2E antibody|P450J antibody|P450RLM6 antibody|xenobiotic monooxygenase antibody
    Gene ID
    1571
    UniProt
    P05181
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