DDB1 antibody (AA 1011-1140)

Catalog No. ABIN3043823

Product Details anti-DDB1 Antibody

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Binding Specificity: AA 1011-1140

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for DNA damage-binding protein 1(DDB1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for DNA damage-binding protein 1(DDB1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: damage-specific DNA binding protein 1, 127 kDa
Protein Name: DNA damage-binding protein 1

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DDB1 recombinant protein (Position: S1011-H1140). Human DDB1 shares 99.2% amino acid (aa) sequence identity with both mouse and rat DDB1.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for DDB1

Target: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Alternative Name: DDB1 (DDB1 Products)

Synonyms: DDBA antibody, UV-DDB1 antibody, XAP1 antibody, XPCE antibody, XPE antibody, XPE-BF antibody, DDB1 antibody, xpe antibody, ddba antibody, xap1 antibody, xpce antibody, xpe-bf antibody, uv-ddb1 antibody, ddb1 antibody, 127kDa antibody, AA408517 antibody, p127-Ddb1 antibody, damage specific DNA binding protein 1 antibody, damage-specific DNA binding protein 1, 127kDa antibody, DNA damage-binding protein 1 antibody, damage-specific DNA binding protein 1 antibody, DDB1 antibody, ddb1 antibody, LOC100187356 antibody, Ddb1 antibody, ddb-1 antibody

Background: The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. And this protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.

Synonyms: Damage specific DNA binding protein 1 antibody|Damage-specific DNA-binding protein 1 antibody|DDB 1 antibody|DDB p127 subunit antibody|Ddb1 antibody|DDB1_HUMAN antibody|DDBa antibody|DNA damage binding protein 1 antibody|DNA damage-binding protein 1 antibody| DNA damage-binding protein a antibody|HBV X-associated protein 1 antibody|UV damaged DNA binding factor antibody|UV damaged DNA binding protein 1 antibody|UV DDB 1 antibody|UV DDB1 antibody|UV-damaged DNA-binding factor antibody|UV-damaged DNA-binding protein 1 antibody|UV-DDB 1 antibody|UV-DDB1 antibody|X associated protein 1 antibody|XAP 1 antibody|XAP-1 antibody|XAP1 antibody|Xeroderma pigmentosum group E complementing protein antibody|Xeroderma pigmentosum group E-complementing protein antibody|XPCE antibody|XPE antibody|XPE BF antibody|XPE binding factor antibody|XPE-BF antibody|XPE-binding factor antibody

Gene ID: 1642

UniProt: Q16531

Pathways: DNA Damage Repair