GFAP antibody (AA 93-432)

Catalog No. ABIN3043832

Product Details anti-GFAP Antibody

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Binding Specificity: AA 93-432

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Glial fibrillary acidic protein(GFAP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Glial fibrillary acidic protein(GFAP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: glial fibrillary acidic protein
Protein Name: Glial fibrillary acidic protein

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GFAP recombinant protein (Position: Q93-M432). Human GFAP shares 94% amino acid (aa) sequence identity with both mouse and rat GFAP.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for GFAP is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-GFAP antibody (ABIN3043832)

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Liu, Zhao, Ji, Zhu, Sui, Knight, Burnstock, He, Yuan, Xiang: "Inhibition of P2X7 receptors improves outcomes after traumatic brain injury in rats." in: Purinergic signalling, Vol. 13, Issue 4, pp. 529-544, (2018) (PubMed).

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Daugherty, Chechneva, Mayrhofer, Deng: "The hGFAP-driven conditional TSPO knockout is protective in a mouse model of multiple sclerosis." in: Scientific reports, Vol. 6, pp. 22556, (2017) (PubMed).

Mi, Gao, Liu, Ye, Li, Jin, Yang, Yang: "Cyclin-dependent kinase inhibitor flavopiridol promotes remyelination in a cuprizone induced demyelination model." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 20, pp. 2780-91, (2017) (PubMed).

Zhai, Zhao, Zhou, Lu, Zhang, Na, Feng, Qiang, Du: "The association and significance of H3K27me3 and a folate metabolic gene ACat2 in neural tube defects." in: Nutrition journal, Vol. 15, Issue 1, pp. 95, (2017) (PubMed).

Xu, Lu, Miao, Wu, -Z Jiang, Ge, Fang, Zhu, Chen, Zhou, Lu, Tang, Wang: "Immunoregulatory effect of neuronal-like cells in inducting differentiation of bone marrow mesenchymal stem cells." in: European review for medical and pharmacological sciences, Vol. 20, Issue 24, pp. 5041-5048, (2017) (PubMed).

Yang, Xie, Hu, Jiang, Shen, Schachner, Zhao: "CHL1 Is Expressed and Functions as a Malignancy Promoter in Glioma Cells." in: Frontiers in molecular neuroscience, Vol. 10, pp. 324, (2017) (PubMed).

Jiang, Luo, Qin, Ma, Li, Zhan, Zhang: "Electroacupuncture Suppresses the NF-κB Signaling Pathway by Upregulating Cylindromatosis to Alleviate Inflammatory Injury in Cerebral Ischemia/Reperfusion Rats." in: Frontiers in molecular neuroscience, Vol. 10, pp. 363, (2017) (PubMed).

Wang, Lin, Cao, Duan, Guan, Xu, Zhu, Xia: "Up-regulation of Interleukin-21 Contributes to Liver Pathology of Schistosomiasis by Driving GC Immune Responses and Activating HSCs in Mice." in: Scientific reports, Vol. 7, Issue 1, pp. 16682, (2017) (PubMed).

Tang, Luo, Qiu, Zhang, Lu, Huang, Xu, Xu: "Interactions between GHRH and GABAARs in the brains of patients with epilepsy and in animal models of epilepsy." in: Scientific reports, Vol. 7, Issue 1, pp. 18110, (2017) (PubMed).

Yuan, Hu, Gao: "Brain Localization and Neurotoxicity Evaluation of Polysorbate 80-Modified Chitosan Nanoparticles in Rats." in: PLoS ONE, Vol. 10, Issue 8, pp. e0134722, (2016) (PubMed).

Cui, Li, Gai, She, Zhang, Xu, Chen, An, Ma, Wang: "Chronic Noise Exposure Acts Cumulatively to Exacerbate Alzheimer's Disease-Like Amyloid-β Pathology and Neuroinflammation in the Rat Hippocampus." in: Scientific reports, Vol. 5, pp. 12943, (2016) (PubMed).

Liao, Jiang, Wang, Zhao, Chen, Li, Wang, Jie, Zhou, Zhang, Chen, Hu: "Histidine provides long-term neuroprotection after cerebral ischemia through promoting astrocyte migration." in: Scientific reports, Vol. 5, pp. 15356, (2016) (PubMed).

Zhu, Li, Luo, Wang, Li, Xie, Gao, Song, Su, Liang et al.: "Reversion of malignant phenotypes of human glioblastoma cells by β-elemene through β-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related ..." in: Journal of translational medicine, Vol. 13, pp. 356, (2016) (PubMed).

Hu, Zhang, Tian, Xun, Wang, Lv: "Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro." in: Molecular medicine reports, Vol. 13, Issue 1, pp. 49-58, (2016) (PubMed).

Xiao, Zhang, Zhang, Wang, Chen, Chen, Lv, Shi, Dong: "Re-infection of the prion from the scrapie‑infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c." in: International journal of molecular medicine, Vol. 37, Issue 3, pp. 716-26, (2016) (PubMed).

Yang, Li, Li, Feng, Liu, Han, Zhang, Qin, Zhang, Nie, Jin: "Effect of Corilagin on the Proliferation and NF-κB in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 1418309, (2016) (PubMed).

Zeng, Zhao, Ouyang, Zhao, Zhang, Chen, Yu, Lei: "Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium." in: Oncology letters, Vol. 12, Issue 2, pp. 815-824, (2016) (PubMed).

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP (GFAP Products)

Synonyms: GFAP antibody, AI836096 antibody, cb345 antibody, etID36982.3 antibody, gfapl antibody, wu:fb34h11 antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody, glial fibrillary acidic protein antibody, GFAP antibody, LOC100136168 antibody, gfap antibody, Gfap antibody

Background: Glial fibrillary acidic protein (GFAP) is a protein that is encoded by the GFAP gene in humans. It is an intermediate filament(IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, and ependymal cells. It is mapped to 17q21.31. GFAP is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell's cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells. This gene has been shown to play a role in mitosis by adjusting the filament network present in the cell. GFAP is necessary for many critical roles in the CNS. What's more, GFAP also plays a role in astrocyte-neuron interactions as well as cell-cell communication.

Synonyms: Astrocyte antibody|FLJ42474 antibody|FLJ45472 antibody|GFAP antibody|GFAP_HUMAN antibody|Glial Fibrillary Acidic Protein antibody|Intermediate filament protein antibody

Gene ID: 2670

UniProt: P14136