MME antibody (AA 52-750)

Catalog No. ABIN3043879

Product Details anti-MME Antibody

Target: MME (Membrane Metallo-Endopeptidase (MME))

Binding Specificity: AA 52-750

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MME antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Neprilysin(MME) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Neprilysin(MME) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: membrane metallo-endopeptidase
Protein Name: Neprilysin

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CD10 recombinant protein (Position: Y52-W750). Human CD10 shares 94% amino acid (aa) sequences identity with both mouse and rat CD10.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for CD10 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-MME antibody (ABIN3043879)

Wang, Tian, Xiao, Zhang, Sun, Tang: "A metanephric adenoma of the kidney associated with polycythemia: A case report." in: Oncology letters, Vol. 11, Issue 1, pp. 352-354, (2016) (PubMed).

Meng, Li, Geng, Ohe, Yu, E, Ye, Yang, Kato, Zhang, Ishida, Ohta, Jin, Kakehata, Geng, Yamakawa: "Decreased expression of follicular dendritic cell-secreted protein correlates with increased immunoglobulin A production in the tonsils of individuals with immunoglobulin A nephropathy." in: Translational research : the journal of laboratory and clinical medicine, Vol. 166, Issue 3, pp. 281-91, (2015) (PubMed).

Target Details for MME

Target: MME (Membrane Metallo-Endopeptidase (MME))

Alternative Name: MME (MME Products)

Synonyms: CALLA antibody, CD10 antibody, NEP antibody, SFE antibody, 6030454K05Rik antibody, C85356 antibody, Nep antibody, neprilysin antibody, membrane metalloendopeptidase antibody, membrane metallo endopeptidase antibody, membrane metallo-endopeptidase antibody, MME antibody, Mme antibody

Target Type: Chemical

Background: CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), Neprilysin, or common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

Synonyms: Atriopeptidase antibody|CALLA antibody|CD10 antibody|CD10 antigen antibody|Common acute lymphocytic leukemia antigen antibody|DKFZp686O16152 antibody|EC 3.4.24.11 antibody|Enkephalinase antibody|EPN antibody|gp100 antibody|Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody|Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody|Membrane metallo endopeptidase antibody|Membrane metallo endopeptidase variant 1 antibody|Membrane metallo endopeptidase variant 2 antibody|Membrane metalloendopeptidase antibody|Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody|Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody|Membrane metalloendopeptidase variant 1 antibody|Membrane metalloendopeptidase variant 2 antibody|MGC126681 antibody|MGC126707 antibody|MME antibody|NEP antibody|NEP_HUMAN antibody|Neprilysin antibody|Neutral endopeptidase 24.11 antibody|Neutral endopeptidase antibody|SFE antibody|Skin fibroblast elastase antibody

Gene ID: 4311

UniProt: P08473

Pathways: RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration