PTH1R antibody (C-Term)

Catalog No. ABIN3043983

Product Details anti-PTH1R Antibody

Target: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Binding Specificity: AA 388-406, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTH1R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for Parathyroid hormone/parathyroid hormone-related peptide receptor(PTH1R) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequence: KLRETNAGRC DTRQQYRKL

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Parathyroid hormone/parathyroid hormone-related peptide receptor(PTH1R) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: parathyroid hormone 1 receptor
Protein Name: Parathyroid hormone/parathyroid hormone-related peptide receptor

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Parathyroid Hormone Receptor 1(388-406aa KLRETNAGRCDTRQQYRKL), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-PTH1R antibody (ABIN3043983)

Ma, Wu, Jin: "The association between parathyroid hormone 1‑34 and the Wnt/β‑catenin signaling pathway in a rat model of osteoarthritis." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 8799-8807, (2018) (PubMed).

Wang, Wang, Dai, Chen, Yang, Dai, Ou, Wang, Lin: "Effects of Intermittent Administration of Parathyroid Hormone (1-34) on Bone Differentiation in Stromal Precursor Antigen-1 Positive Human Periodontal Ligament Stem Cells." in: Stem cells international, Vol. 2016, pp. 4027542, (2016) (PubMed).

Li, He, Yao, Song, Zeng, Peng, Rosol, Deng: "TGF-? Induces Degradation of PTHrP Through Ubiquitin-Proteasome System in Hepatocellular Carcinoma." in: Journal of Cancer, Vol. 6, Issue 6, pp. 511-8, (2015) (PubMed).

Target Details for PTH1R

Target: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Alternative Name: PTH1R (PTH1R Products)

Synonyms: PFE antibody, PTHR antibody, PTHR1 antibody, PPR antibody, Pthr antibody, Pthr1 antibody, pthr1 antibody, zPTH1R antibody, PTHrel antibody, PTH1 antibody, PTH antibody, parathyroid hormone 1 receptor antibody, parathyroid hormone 1 receptor a antibody, PTH1R antibody, Pth1r antibody, pth1ra antibody

Background: Parathyroid hormone/parathyroid hormone-related peptide receptor, also known as PTH1R, PTHR, is a protein that in humans is encoded by the PTH1R gene. PTHR is a member of a family of G protein-coupled receptors. PTHR gene was mapped to the vicinity of the 3p21.3-p21.2 boundary by PCR analysis of human/rodent somatic cell hybrid panels using oligonucleotide primers designed to amplify a portion of the gene from genomic DNA. This is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. PTH1R functions as a receptor for parathyroid hormone and for parathyroid hormone-related protein.

Synonyms: MGC138426 antibody|MGC138452 antibody|Parathyroid hormone 1 receptor antibody|Parathyroid hormone/parathyroid hormone related peptide receptor antibody|Parathyroid hormone/parathyroid hormone related protein receptor antibody|Parathyroid hormone/parathyroid hormone-related peptide receptor antibody|PTH receptor antibody|PTH/PTHr receptor antibody|PTH/PTHrP receptor antibody|PTH/PTHrP type I receptor antibody|PTH1 receptor antibody|PTH1R antibody|PTH1R_HUMAN antibody|PTHR 1 antibody|PTHR antibody|PTHR1 antibody

UniProt: Q03431

Pathways: Regulation of Carbohydrate Metabolic Process