SLC1A3 antibody (C-Term)
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- Target See all SLC1A3 Antibodies
- SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))
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Binding Specificity
- AA 519-537, C-Term
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Reactivity
- Rat, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SLC1A3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Purpose
- Rabbit IgG polyclonal antibody for Excitatory amino acid transporter 1(SLC1A3) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
- Sequence
- MKKPYQLIAQ DNETEKPID
- Cross-Reactivity (Details)
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Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - Characteristics
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Rabbit IgG polyclonal antibody for Excitatory amino acid transporter 1(SLC1A3) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: solute carrier family 1(glial high affinity glutamate transporter), member 3
Protein Name: Excitatory amino acid transporter 1 - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-terminus of human EAAT1(519-537aa MKKPYQLIAQDNETEKPID), different from the related rat and mouse sequences by three amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product SLC1A3 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Thimerosal (Merthiolate), Sodium azide
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Expiry Date
- 12 months
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Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes." in: Stem cell reports, Vol. 9, Issue 2, pp. 615-628, (2018) (PubMed).
: "Tumor necrosis factor-? promotes the expression of excitatory amino-acid transporter 2 in astrocytes: Optimal concentration and incubation time." in: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1909-1913, (2014) (PubMed).
: "
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Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes." in: Stem cell reports, Vol. 9, Issue 2, pp. 615-628, (2018) (PubMed).
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- Target
- SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))
- Alternative Name
- SLC1A3 (SLC1A3 Products)
- Background
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Solute carrier family 1(glial high-affinity glutamate transporter), member 3, also known as SLC1A3, EAAT1 or GLAST, is a protein that in humans is encoded by the SLC1A3 gene. This gene is a member of high affinity glutamate transporter family. SLC1A3 is mapped to chromosome 5p13.2 by fluorescence in situ hybridization(FISH). This gene transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. This gene acts as a symport by cotransporting sodium.
Synonyms: EA6 antibody|EAA1_HUMAN antibody|EAAT1 antibody|Excitatory amino acid transporter 1 antibody|FLJ25094 antibody|GLAST antibody|GLAST-1 antibody|GLAST1 antibody|Glial high affinity glutamate transporter antibody|glutamate/aspartate transporter, high affinity, sodium-dependent antibody|High affinity neuronal glutamate transporter antibody|Slc1a3 antibody|Sodium dependent glutamate/aspartate transporter antibody|Sodium-dependent glutamate/aspartate transporter 1 antibody|Solute carrier family 1(glial high affinity glutamate transporter) member 3 antibody|Solute carrier family 1 member 3 antibody - UniProt
- P43003
- Pathways
- Sensory Perception of Sound, Synaptic Membrane, Dicarboxylic Acid Transport
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