Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 Interacting Protein (APBB1IP) (AA 647-666), (C-Term) antibody

Catalog No. ABIN3044029

Product Details

Target: Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 Interacting Protein (APBB1IP)

Binding Specificity: AA 647-666, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Amyloid beta A4 precursor protein-binding family B member 1-interacting protein(APBB1IP) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Rat.

Sequence: EQDFMSDLMK ALQKKRGNVS

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Amyloid beta A4 precursor protein-binding family B member 1-interacting protein(APBB1IP) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Rat.
Gene Name: amyloid beta(A4) precursor protein-binding, family B, member 1 interacting protein
Protein Name: Amyloid beta A4 precursor protein-binding family B member 1-interacting protein

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human APBB1IP(647-666aa EQDFMSDLMKALQKKRGNVS), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Amyloid beta (A4) Precursor Protein-Binding, Family B, Member 1 Interacting Protein (APBB1IP)

Alternative Name: APBB1IP (APBB1IP Products)

Synonyms: zgc:63968 antibody, apbb1ip antibody, MGC80693 antibody, APBB1IP antibody, INAG1 antibody, PREL1 antibody, RARP1 antibody, RIAM antibody, 9930118P07Rik antibody, Prp48 antibody, amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein antibody, amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein S homeolog antibody, amyloid beta precursor protein binding family B member 1 interacting protein antibody, amyloid beta A4 precursor protein-binding family B member 1-interacting protein antibody, apbb1ip antibody, apbb1ip.S antibody, APBB1IP antibody, LOC707383 antibody, LOC100439620 antibody, LOC100598478 antibody, Apbb1ip antibody

Background: APBB1IP(APBB1-Interacting Protein), also called RIAM or RARP1, is a protein that in humans is encoded by the APBB1IP gene. By genomic sequence analysis, Lafuente et al.(2004) mapped the RIAM gene to chromosome 10p12.1. Using promoter-reporter gene assays, Inagaki et al.(2003) found that RARP1 suppressed transcription from AP1 and SRE sites, but not CRE sites, in all cell lines examined. The proline-rich regions of RARP1 suppressed AP1 transactivation. Lafuente et al.(2004) found that RIAM interacted with profilin and VASP, molecules that regulate actin dynamics, as well as with RAP1-GTP.

Synonyms: AB1IP antibody|Amyloid beta A4 precursor protein binding family B member 1 interacting protein antibody|Amyloid beta precursor protein binding family B member 1 antibody|APBB1 interacting protein 1 antibody|OTTHUMP00000019344 antibody|PREL 1 antibody|PREL1 antibody|Proline rich EVH1 ligand 1 antibody|Proline rich protein 73 antibody|Rap1 GTP interacting adapter molecule antibody|RAP1 interacting adaptor molecule antibody|RARP 1 antibody|RARP1 antibody|Retinoic acid responsive proline rich protein 1 antibody

UniProt: Q7Z5R6