TSC1 antibody (C-Term)
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- Target See all TSC1 Antibodies
- TSC1 (Tuberous Sclerosis 1 (TSC1))
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Binding Specificity
- AA 1150-1164, C-Term
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Reactivity
- Human, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This TSC1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Hamartin(TSC1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Sequence
- QLHIMDYNET HHEHS
- Cross-Reactivity (Details)
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Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - Characteristics
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Rabbit IgG polyclonal antibody for Hamartin(TSC1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: tuberous sclerosis 1
Protein Name: Hamartin - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-terminus of human Hamartin(1150-1164aa QLHIMDYNETHHEHS), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
- Isotype
- IgG
- Top Product
- Discover our top product TSC1 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for TSC1 is approximately 5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Thimerosal (Merthiolate), Sodium azide
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Expiry Date
- 12 months
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- Target
- TSC1 (Tuberous Sclerosis 1 (TSC1))
- Alternative Name
- TSC1 (TSC1 Products)
- Synonyms
- LAM antibody, TSC antibody, hamartin antibody, mKIAA0243 antibody, tsc1 antibody, wu:fc38b04 antibody, fa99f04 antibody, wu:fa99f04 antibody, zgc:63657 antibody, TSC complex subunit 1 antibody, tuberous sclerosis 1 antibody, TSC complex subunit 1b antibody, TSC complex subunit 1a antibody, TSC1 antibody, Tsc1 antibody, tsc1b antibody, tsc1 antibody, tsc1a antibody
- Background
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TSC1(Tuberous Sclerosis 1), also called HAMARTIN or TSC, is a human protein and gene. As part of a comprehensive strategy to identify the gene mutant in tuberous sclerosis-1, van Slegtenhorst et al.(1997) developed an overlapping contig of clones from the 1.4-Mb TSC1 region on chromosome 9. Benvenuto et al.(2000) showed that overexpression of the TSC1 gene in rat fibroblasts inhibits growth and causes changes in cell morphology. Van Slegtenhorst et al.(1998) showed that hamartin and tuberin associate physically in vivo, however, and that the interaction is mediated by predicted coiled-coil domains.
Synonyms: Hamartin antibody|kiaa0243 antibody|LAM antibody|TSC antibody|TSC1 antibody|Tsc1 gene antibody|TSC1_HUMAN antibody|Tuberous sclerosis 1 antibody|Tuberous sclerosis 1 protein antibody - UniProt
- Q92574
- Pathways
- RTK Signaling, AMPK Signaling, Regulation of Cell Size, Tube Formation
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