PGK1 antibody (Middle Region)

Catalog No. ABIN3044064

Product Details anti-PGK1 Antibody

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Binding Specificity: AA 166-180, Middle Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PGK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for Phosphoglycerate kinase 1(PGK1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: FGTAHRAHSS MVGVN

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Phosphoglycerate kinase 1(PGK1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: phosphoglycerate kinase 1
Protein Name: Phosphoglycerate kinase 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PGK1(166-180aa FGTAHRAHSSMVGVN), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for PGK1 is approximately 1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Rat, Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PGK1

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Alternative Name: PGK1 (PGK1 Products)

Synonyms: MIG10 antibody, PGKA antibody, Pgk-1 antibody, Pgk antibody, PGK antibody, wu:fd59b07 antibody, wu:fj36g06 antibody, zgc:56252 antibody, zgc:77899 antibody, PGK1 antibody, phosphoglycerate kinase 1 antibody, pgk2 antibody, pgka antibody, phosphoglycerate kinase 1 antibody, phosphoglycerate kinase 1 L homeolog antibody, PGK1 antibody, Pgk1 antibody, pgk1 antibody, LOC100348124 antibody, pgk1.L antibody

Background: PGK1 (Phosphoglycerate Kinase 1), also known as PGKA, is an enzyme that in humans is encoded by the PGK1 gene. The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. And the encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.

Synonyms: Cell migration-inducing gene 10 protein antibody|MGC117307 antibody|MGC8947 antibody|MIG10 antibody|pgk1 antibody|PGK1_HUMAN antibody|PGKA antibody|Phosphoglycerate kinase 1 antibody|Primer recognition protein 2 antibody|PRP 2 antibody

UniProt: P00558

Pathways: Cellular Glucan Metabolic Process