ABCG1 antibody (C-Term)

Catalog No. ABIN3044094

Product Details anti-ABCG1 Antibody

Target: ABCG1 (ATP-Binding Cassette, Sub-Family G (WHITE), Member 1 (ABCG1))

Binding Specificity: AA 627-647, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCG1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for ATP-binding cassette sub-family G member 1(ABCG1) detection. Tested with WB in Human,Mouse,Rat.

Sequence: ETCHFQKSEA ILRELDVENA K

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for ATP-binding cassette sub-family G member 1(ABCG1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: ATP-binding cassette, sub-family G(WHITE), member 1
Protein Name: ATP-binding cassette sub-family G member 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ABCG1(627-647aa ETCHFQKSEAILRELDVENAK), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, The detection limit for ABCG1 is approximately 5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-ABCG1 antibody (ABIN3044094)

Shang, Yu, Wang, Chen, Fang, Yang, Zhou, Nie, Zhou, Zhou: "Fibroblast growth factor 21 enhances cholesterol efflux in THP-1 macrophage-derived foam cells." in: Molecular medicine reports, Vol. 11, Issue 1, pp. 503-8, (2014) (PubMed).

Yan, Ding, Dong, Li, Wu: "Sphingomyelin synthase overexpression increases cholesterol accumulation and decreases cholesterol secretion in liver cells." in: Lipids in health and disease, Vol. 10, pp. 46, (2011) (PubMed).

Target Details for ABCG1

Target: ABCG1 (ATP-Binding Cassette, Sub-Family G (WHITE), Member 1 (ABCG1))

Alternative Name: ABCG1 (ABCG1 Products)

Synonyms: ABCG1 antibody, zgc:162197 antibody, ABC8 antibody, WHITE1 antibody, AW413978 antibody, Abc8 antibody, White antibody, ATP binding cassette subfamily G member 1 antibody, ATP-binding cassette, sub-family G (WHITE), member 1 antibody, ABCG1 antibody, abcg1 antibody, Abcg1 antibody

Background: ABCG1(Atp-binding cassette, subfamily g, member 1) also known as ABC8, ABC TRANSPORTER 8 or WHITE, DROSOPHILA, HOMOLOG OF, is a protein that in humans is encoded by the ABCG1 gene. ABCG1 is a regulator of macrophage cholesterol and phospholipid transport. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette(ABC) transporters.The ABCG1 gene is mapped on 21q22.3. This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. ABCG1 is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. Inhibition of ABCG1 protein expression using an antisense strategy resulted in reduced HDL3-dependent efflux of cholesterol and choline-phospholipids. In macrophages, silencing of GPS2 by RNA interference reduced ABCG1 expression and diminished ABCG1-mediated cholesterol efflux.

Synonyms: ABC transporter 8 antibody|Abc8 antibody|ABCG1 antibody|ABCG1_HUMAN antibody|ATP-binding cassette sub family G member 1 antibody|ATP-binding cassette sub-family G member 1 antibody|ATP-binding cassette transporter 8 antibody|ATP-binding cassette transporter member 1 of subfamily G antibody|ATP-binding cassette, sub family G WHITE member 1 antibodyhomolog of Drosophila white antibody|MGC34313 antibody|White protein homolog antibody|White protein homolog ATP binding cassette transporter 8 antibody|WHITE1 antibody|wht1 antibody

UniProt: P45844

Pathways: Lipid Metabolism