CD2 antibody (Middle Region)
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- Target See all CD2 Antibodies
- CD2
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Binding Specificity
- AA 224-242, Middle Region
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Reactivity
- Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CD2 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Purpose
- Rabbit IgG polyclonal antibody for T-cell surface antigen CD2(CD2) detection. Tested with WB in Mouse,Rat.
- Sequence
- FIFCICKRRK RNRRRKDEE
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for T-cell surface antigen CD2(CD2) detection. Tested with WB in Mouse,Rat.
Gene Name: CD2 Molecule
Protein Name: T-cell surface antigen CD2 - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence in the middle region of mouse CD2(224-242aa FIFCICKRRKRNRRRKDEE), different from the related rat sequence by two amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product CD2 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Thimerosal (Merthiolate), Sodium azide
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Expiry Date
- 12 months
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- Target
- CD2
- Alternative Name
- CD2 (CD2 Products)
- Synonyms
- CD2 antibody, LFA-2 antibody, Ly-37 antibody, Ly37 antibody, SRBC antibody, T11 antibody, CD2R antibody, LFA2 antibody, OX-34 antibody, OX34 antibody, CD2 molecule antibody, CD2 antigen antibody, Cd2 molecule antibody, CD2 antibody, Cd2 antibody
- Background
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CD2(cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer(NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. It is one of the earliest T-cell markers, being present on more than 95 % of thymocytes, it is also found on some natural killer cells but not on B lymphocytes. Due to its structural characteristics, CD2 is a member of the immunoglobulin superfamily, it possesses two immunoglobulin-like domains in its extracellular portionThe localization of CD2 to 1p13 was established by in situ hybridization. By Southern blotting of DNA from a panel of somatic cell hybrids, Clayton et al.(1988) assigned the CD2 gene to human chromosome 1 and murine chromosome 3.CD2 interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3(LFA-3/CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. With the use of transgenic mice, such an LCR was identified within the 3-prime flanking region of the human CD2 gene.
Synonyms: CD 2 antibody|CD2 antibody|CD2 antigen(p50), sheep red blood cell receptor antibody|CD2 antigen antibody|CD2 Molecule antibody|CD2_HUMAN antibody|Erythrocyte receptor antibody|FLJ46032 antibody|LFA-2 antibody|LFA-3 receptor antibody|LFA2 antibody|LFA3 receptor antibody|Ly-37 antibody|Lymphocyte function antigen 2 antibody|lymphocyte-function antigen-2 antibody|OTTHUMP00000024366 antibody|Rosette receptor antibody|SRBC antibody|T cell surface antigen CD2 antibody|T-cell surface antigen CD2 antibody|T-cell surface antigen T11/Leu-5 antibody|T-lymphocyte surface CD2 antigen antibody|T11 antibody - UniProt
- P08920
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