E2F6 antibody (Middle Region)

Catalog No. ABIN3044189

Product Details anti-E2F6 Antibody

Target: E2F6 (E2F Transcription Factor 6 (E2F6))

Binding Specificity: AA 162-177, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F6 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Transcription factor E2F6(E2F6) detection. Tested with WB in Human,Mouse,Rat.

Sequence: KDCAQQLFEL TDDKEN

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Transcription factor E2F6(E2F6) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: E2F transcription factor 6
Protein Name: Transcription factor E2F6(E2F-6)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human E2F6(162-177aa KDCAQQLFELTDDKEN), different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for E2F6

Target: E2F6 (E2F Transcription Factor 6 (E2F6))

Alternative Name: E2F6 (E2F6 Products)

Synonyms: E2F-6 antibody, AI462434 antibody, E2F6b antibody, EMA antibody, E2F transcription factor 6 antibody, E2F6 antibody, E2f6 antibody

Background: E2F6(E2F transcription factor 6) also known as E2F-6. Northern blot analysis revealed that E2F6 is expressed as 2 mRNAs, 2.5 and 3.5 kb, in all human tissues and cell lines tested. The predicted human and mouse E2F6 protein sequences are 92 % identical. The DNA-binding and dimerization domains of E2F6 are highly related to those of other E2F family members, but this protein lacks the sequences necessary for either transcriptional activation or binding to RB1, p107, or p130. E2F6 can act to repress the transcription of E2F-responsive genes by countering the activity of other E2F complexes. E2F6 contributes to gene silencing in a manner independent of retinoblastoma protein family members. E2F6 is found in a multimeric protein complex that contains MGA and MAX, and thus the complex can bind not only to the E2F binding site but also to MYC- and Brachyury- binding sites. The E2F6 complex preferentially occupies target promoters in G0 cells rather than in G1 cells.

Synonyms: E2F 6 antibody|E2F binding site modulating activity protein antibody|E2F transcription factor 6 antibody|E2F transcription factor 6 isoform 1 antibody|E2F-6 antibody|E2F6 antibody|E2F6_HUMAN antibody|EMA antibody|MGC111545 antibody|Transcription factor E2F6 antibody

UniProt: O75461