SDHC antibody (N-Term)

Catalog No. ABIN3044203

Product Details anti-SDHC Antibody

Target: SDHC (Succinate Dehydrogenase Complex, Subunit C, Integral Membrane Protein, 15kDa (SDHC))

Binding Specificity: AA 35-50, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SDHC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Succinate dehydrogenase cytochrome b560 subunit, mitochondrial(SDHC) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: KEEMERFWNK NIGSNR

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Succinate dehydrogenase cytochrome b560 subunit, mitochondrial(SDHC) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: succinate dehydrogenase complex, subunit C, integral membrane protein, 15 kDa
Protein Name: Succinate dehydrogenase cytochrome b560 subunit, mitochondrial

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SDHC(35-50aa KEEMERFWNKNIGSNR), different from the related mouse and rat sequences by three amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SDHC

Target: SDHC (Succinate Dehydrogenase Complex, Subunit C, Integral Membrane Protein, 15kDa (SDHC))

Alternative Name: SDHC (SDHC Products)

Background: SDHC(succinate dehydrogenase complex, subunit C, integral membrane protein, 15 kDa) also known as SDH3, SUCCINATE DEHYDROGENASE CYTOCHROME b. The SDHC gene contains 6 exons. Oostveen et al.(1995) found that in fact it was protein from the bovine SDH3 gene(encoding 1 of the 2 integral membrane proteins) that complemented the hamster mutation. The authors localized the human SDH3 gene to the short arm of chromosome one, within 1 to 2 Mb from the centromere. Additionally, the authors stated that Southern analyses of human genomic DNA suggested that there are multiple SDH3 genes or pseudogenes. Elbehti-Green et al.(1998) confirmed the assignment of the SDHC gene to 1q21 by FISH. Baysal et al.(2004) described a family with PGL3 in which an 8,372-bp deletion in the SDHC gene was transmitted both maternally and paternally, without evidence of genomic imprinting.

Synonyms: CYB560 antibody|C560_HUMAN antibody|CYBL antibody|Cytochrome B large subunit of complex II antibody|Integral membrane protein CII-3 antibody|mitochondrial antibody|PGL3 antibody|QPs-1 antibody|QPs1 antibody|SDH3 antibody|sdhC antibody|Succinate dehydrogenase complex subunit C antibody|Succinate dehydrogenase complex subunit C integral membrane protein 15 kDa antibody|Succinate dehydrogenase cytochrome b560 subunit antibody|Succinate dehydrogenase cytochrome b560 subunit mitochondrial antibody|Succinate dehydrogenase cytochrome b560 subunit, mitochondrial precursor antibody|Succinate-ubiquinone oxidoreductase cytochrome B large subunit antibody

UniProt: Q99643