Arrestin 3 antibody (C-Term)

Catalog No. ABIN3044208

Product Details anti-Arrestin 3 Antibody

Target: Arrestin 3 (ARRB2) (Arrestin, beta 2 (ARRB2))

Binding Specificity: AA 395-409, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Arrestin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Beta-arrestin-2(ARRB2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: RLKGMKDDDY DDQLC

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Beta-arrestin-2(ARRB2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: arrestin beta-2
Protein Name: Beta-arrestin-2

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Beta Arrestin 2(395-409aa RLKGMKDDDYDDQLC), different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for ARRB2 is approximately 5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Arrestin 3

Target: Arrestin 3 (ARRB2) (Arrestin, beta 2 (ARRB2))

Alternative Name: ARRB2 (ARRB2 Products)

Synonyms: ARRB2 antibody, arb2 antibody, arr2 antibody, arrestin antibody, barr2 antibody, betaarr2 antibody, ARB2 antibody, ARR2 antibody, BARR2 antibody, BARRES antibody, AI326910 antibody, AW122872 antibody, arrb2 antibody, zgc:64007 antibody, arrestin beta 2 antibody, arrestin, beta 2 antibody, arrestin beta 2 L homeolog antibody, arrestin, beta 2b antibody, ARRB2 antibody, arrb2 antibody, Arrb2 antibody, arrb2.L antibody, arrb2b antibody

Background: ARRB2(arrestin beta-2), also known as Beta-arrestin-2, BARR2, ARB2, DKFZp686L0365, arrestin 3, is an intracellularprotein that in humans is encoded by the ARRB2 gene. Members of arrestin/beta-arrestin protein family are though to participate in agonist-mediated desensitization of G protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals, as well as having signalling roles in their own right. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Beta-arrestins were originally discovered in the context of heterotrimeric G protein-coupled receptor desensitization, but they also function in internalization and signaling of these receptors.

Synonyms: ARB 2 antibody|ARB2 antibody|ARR 2 antibody|ARR2 antibody|ARRB 2 antibody|ARRB2 antibody|ARRB2_HUMAN antibody|Arrestin 3 antibody|Arrestin beta 2 antibody|Arrestin beta-2 antibody|BARR2 antibody|Beta-arrestin-2 antibody|DKFZp686L0365 antibody|HGNC:712 antibody

UniProt: P32121

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Phototransduction, Thromboxane A2 Receptor Signaling