ALOX5 antibody (C-Term)

Catalog No. ABIN3044238

Product Details anti-ALOX5 Antibody

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Binding Specificity: AA 650-667, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Arachidonate 5-lipoxygenase(ALOX5) detection. Tested with WB, IHC-P in Human.

Sequence: AERNKKKQLP YYYLSPDR

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Arachidonate 5-lipoxygenase(ALOX5) detection. Tested with WB, IHC-P in Human.
Gene Name: arachidonate 5-lipoxygenase
Protein Name: Arachidonate 5-lipoxygenase(5-LO/5-lipoxygenase)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ALOX5(650-667aa AERNKKKQLPYYYLSPDR), different from the related rat and mouse sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Alternative Name: ALOX5 (ALOX5 Products)

Synonyms: 5-LO antibody, 5-LOX antibody, 5LPG antibody, LOG5 antibody, 5LO antibody, 5LX antibody, AI850497 antibody, F730011J02 antibody, LOX5A antibody, si:dkey-194n13.2 antibody, lox5 antibody, arachidonate 5-lipoxygenase antibody, arachidonate 5-lipoxygenase a antibody, Arachidonate 5-lipoxygenase antibody, ALOX5 antibody, Alox5 antibody, alox5a antibody, AFUA_4G02770 antibody, AOR_1_1362014 antibody, lox5 antibody

Background: ALOX5(ARACHIDONATE 5-LIPOXYGENASE), also known as LOG5 or 5-LO(5-LIPOXYGENASE), is an enzyme that in humans is encoded by the ALOX5 gene. ALOX5 is a member of the lipoxygenase family of enzymes which also transforms EFAs into leukotrienes and is a current target for pharmaceutical intervention in a number of diseases. The enzyme 5-lipoxygenase catalyzes 2 reactions in the formation of leukotrienes. The ALOX5 gene is mapped to chromosome 10q11.21 based on an alignment of the ALOX5 sequence with the genomic sequence. Human 5-LO contains 3 nuclear localization sequences(NLSs) and a phosphorylation site involved in nuclear localization. Compared with age-matched 5-LO competent mice, the 5-LO knockout mice developed less right heart hypertrophy. Pharmacologic inhibition or ALOX5 gene disruption resulted in a significant decrease of beta-amyloid production and gamma-secretase levels. ALOX5 activity is short-lived, apparently in part because of an intrinsic instability of the enzyme.

Synonyms: 5 LO antibody|5 LOX antibody|5-lipoxygenase antibody|5-LO antibody|5-LOX antibody|5LOX antibody|5LPG antibody|ALOX 5 antibody|Alox5 antibody|Arachidonate 5 lipoxygenase antibody|Arachidonate 5-lipoxygenase antibody|arachidonic 5-lipoxygenase alpha-10 isoform antibody|arachidonic 5-lipoxygenase delta-10-13 isoform antibody|arachidonic 5-lipoxygenase delta-13 isoform antibody|arachidonic 5-lipoxygenase delta-p10 isoform antibody|Arachidonic acid 5 lipoxygenase antibody|Leukotriene A4 synthase antibody|LOG 5 antibody|LOG5 antibody|LOX5_HUMAN antibody|MGC163204 antibody

UniProt: P09917