Tenascin R antibody (N-Term)

Catalog No. ABIN3044266

Product Details anti-Tenascin R Antibody

Target: Tenascin R (TNR) (Tenascin R (Restrictin, Janusin) (TNR))

Binding Specificity: AA 104-117, N-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tenascin R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Tenascin-R(TNR) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: QTSDHESQVT FTHK

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Tenascin-R(TNR) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: tenascin R
Protein Name: Tenascin-R(TN-R)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse TNR(104-117aa QTSDHESQVTFTHK), identical to the related rat sequence, and different from the related human sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for TNR is approximately 3 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Tenascin R

Target: Tenascin R (TNR) (Tenascin R (Restrictin, Janusin) (TNR))

Alternative Name: TNR (TNR Products)

Synonyms: TN-R antibody, J1-tenascin antibody, janusin antibody, restrictin antibody, J1NRM antibody, tenascin R antibody, TNR antibody, Tnr antibody

Background: Tenascin-R is a protein that in humans is encoded by the TNR gene. Tenascin-R(TNR) is an extracellular matrix protein expressed primarily in the central nervous system. It is a member of the tenascin(TN) gene family, which includes at least 3 genes in mammals: TNC(or hexabrachion), TNX(TNXB), and TNR. The genes are expressed in distinct tissues at different times during embryonic development and are present in adult tissues. TNR has been detected predominantly in the central nervous system and is localized around motor neurons and on motor axons in the spinal cord, cerebellum, hippocampus, and olfactory bulb. It is suggested that tenascin-R has a role in initiating the detachment of neuroblasts from tangential chains and in initiating radial migration of the cells.

Synonyms: Janusin antibody|MGC149328 antibody|Restrictin antibody|Tenascin R(restrictin, janusin) antibody|Tenascin-R antibody|TENR_HUMAN antibody|TN-R antibody|Tnr antibody

Pathways: Regulation of Cell Size