Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1) (AA 138-158), (N-Term) antibody

Details for Product No. ABIN3044289, Supplier: Log in to see
Antigen
  • APPL
  • DIP13alpha
  • 2900057D21Rik
  • 7330406P05Rik
  • AI585782
  • AW209077
  • BB022931
  • C88264
  • DIP13
  • RGD1309388
  • adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
  • APPL1
  • Appl1
Epitope
AA 138-158, N-Term
15
9
5
4
4
3
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
64
33
20
7
5
3
3
3
3
3
1
1
Host
Rabbit
43
20
3
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
60
33
17
11
3
3
1
1
1
Options
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Purpose Rabbit IgG polyclonal antibody for DCC-interacting protein 13-alpha(APPL1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human APPL(138-158aa NDHDAAINRYSRLSKKRENDK), identical to the related mouse and rat sequences.
Sequence NDHDAAINRY SRLSKKREND K
Isotype IgG
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for DCC-interacting protein 13-alpha(APPL1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Protein Name: DCC-interacting protein 13-alpha(Dip13-alpha)
Purification Immunogen affinity purified.
Alternative Name APPL1 (APPL1 Antibody Abstract)
Background DCC-interacting protein 13-alpha(APPL1) is a protein that in humans is encoded by the APPL1 gene. The APPL1 gene is mapped to 3q21.1-p13.3. It is said to contain 709 amino acids and share 54 % amino acid identity with APPL2. APPL is highly expressed in skeletal muscle, heart, ovary, and pancreas, tissues in which AKT2 mRNA is abundant. It has been regarded as an adaptor that may tether inactive AKT2 to the PI3K in the cytoplasm and thereby may expedite recruitment of AKT2 and PI3K to the cell membrane upon mitogenic stimulation.

Synonyms: Adapter protein containing PH domain antibody|Adaptor Protein Containing pH Domain antibody|Adaptor protein containing pH domain PTB domain and leucine zipper motif 1 antibody|Adaptor protein containing pH domain PTB domain and leucine zipper motif antibody|AKT2 INTERACTOR antibody|APPL 1 antibody|APPL1 antibody|DCC interacting protein 13 alpha antibody|DCC-interacting protein 13-alpha antibody|DIP 13 alpha antibody|DIP13 alpha antibody|Dip13-alpha antibody|DIP13alpha antibody|DP13A_HUMAN antibody|KIAA1428 antibody|PTB domain and leucine zipper motif 1 antibody|PTB Domain and Leucine Zipper Motif antibody|Signaling adaptor protein DIP13alpha antibody
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1) (AA 138-158), (N-Term) antibody (ABIN3044289) Anti-APPL antibody, Western blotting WB: HELA Cell Lysate