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CAPNL1 antibody (Middle Region)

CAPNL1 Reactivity: Human, Mouse, Rat WB, IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3044292
  • Target See all CAPNL1 Antibodies
    CAPNL1 (Calpain 1, Large Subunit (CAPNL1))
    Binding Specificity
    • 10
    • 8
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 312-326, Middle Region
    Reactivity
    • 52
    • 17
    • 17
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 46
    • 12
    • 1
    Rabbit
    Clonality
    • 48
    • 11
    Polyclonal
    Conjugate
    • 36
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This CAPNL1 antibody is un-conjugated
    Application
    • 34
    • 23
    • 20
    • 9
    • 9
    • 9
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Purpose
    Rabbit IgG polyclonal antibody for Calpain-1 catalytic subunit(CAPN1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
    Sequence
    EWNNVDPYER DQLRV
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for Calpain-1 catalytic subunit(CAPN1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
    Gene Name: calpain 1,(mu/I) large subunit
    Protein Name: Calpain-1 catalytic subunit
    Purification
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1(312-326aa EWNNVDPYERDQLRV), different from the mouse sequence by two amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product CAPNL1 Primary Antibody
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
    ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Preservative
    Thimerosal (Merthiolate), Sodium azide
    Precaution of Use
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    Expiry Date
    12 months
  • Ye, Wang, Zhang, Song, Yang, Li, Li, Su, Yang, Ma: "Over-expression of calpastatin inhibits calpain activation and attenuates post-infarction myocardial remodeling." in: PLoS ONE, Vol. 10, Issue 3, pp. e0120178, (2015) (PubMed).

    Chen, Tang, Gao, Xu, Jiang, Cao, Fu, Sun, Liu, Shi: "Fibrosis, adipogenesis, and muscle atrophy in congenital muscular torticollis." in: Medicine, Vol. 93, Issue 23, pp. e138, (2014) (PubMed).

    Mo, Chen, Li, Zheng, Ke, Deng, Li, Jiang, Wu, Wang, Wang, Yang, Cao: "Suppression of NHE1 by small interfering RNA inhibits HIF-1?-induced angiogenesis in vitro via modulation of calpain activity." in: Microvascular research, Vol. 81, Issue 2, pp. 160-8, (2011) (PubMed).

  • Target
    CAPNL1 (Calpain 1, Large Subunit (CAPNL1))
    Alternative Name
    CAPN1 (CAPNL1 Products)
    Synonyms
    CANP antibody, CANP1 antibody, CANPL1 antibody, muCANP antibody, muCL antibody, Capa-1 antibody, Capa1 antibody, mu-calpin antibody, si:ch211-203k12.4 antibody, si:dkeyp-101g1.3 antibody, calpain antibody, calpain 1 antibody, calpain 1, (mu/I) large subunit antibody, calpain 1, (mu/I) large subunit b antibody, calpain 11 antibody, CAPN1 antibody, Capn1 antibody, capn1 antibody, capn1b antibody, CAPN11 antibody
    Background
    Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I(mu-calpain) and calpain II(m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L(large, catalytic, 80 kD) and S(small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits, L1(CAPN1) and L2. By quantitative RT-PCR, Ueyama et al.(1998) found that expression of calpain-1 and calpain-2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy(e.g., DMD, 310200) and 2 men and 3 women with amyotrophic lateral sclerosis(ALS, 105400) compared with controls. Using cDNA clones as probes, Ohno et al.(1989, 1990) assign CANPL1 to chromosome 11.

    Synonyms: Ca2 activated neutral protease antibody|Calcium activated neutral proteinase 1 antibody|Calcium activated neutral proteinase antibody|Calcium activated neutral proteinase small subunit antibody|Calcium dependent protease small subunit 1 antibody|Calcium dependent protease small subunit antibody|Calcium-activated neutral proteinase 1 antibody|Calpain 1(mu/I) large subunit antibody|Calpain 1 antibody|Calpain 1 catalytic subunit antibody|Calpain 1 large subunit antibody|Calpain Large Polypeptide L1 antibody|Calpain Large Polypeptide L1 antibody|Calpain mu type antibody|Calpain mu-type antibody|Calpain regulatory subunit antibody|Calpain small subunit 1 antibody|Calpain-1 catalytic subunit antibody|Calpain-1 large subunit antibody|Calpain1 antibody|CAN1_HUMAN antibody|CANP 1 antibody|CANP antibody|CANP small subunit antibody|CANPL 1 antibody|CANPL1 antibody|CAPN 1 antibody|CAPN1 antibody|Cell proliferation inducing protein 30 antibody|Cell proliferation-inducing gene 30 protein antibody|Micromolar Calpain antibody|Micromolar-calpain antibody|Mu Calpain antibody|muCANP antibody|muCL antibody
    UniProt
    P07384
    Pathways
    Apoptosis
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