Apoptosis Inhibitor 5 antibody (C-Term)

Catalog No. ABIN3044316

Product Details anti-Apoptosis Inhibitor 5 Antibody

Target: Apoptosis Inhibitor 5 (API5)

Binding Specificity: AA 487-504, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Apoptosis Inhibitor 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Rabbit IgG polyclonal antibody for Apoptosis inhibitor 5(API5) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Sequence: KYSSNLGNFN YERSLQGK

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Apoptosis inhibitor 5(API5) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: apoptosis inhibitor 5
Protein Name: Apoptosis inhibitor 5(API-5)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Apoptosis inhibitor 5(487-504aa KYSSNLGNFNYERSLQGK)(b), different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for Apoptosis Inhibitor 5

Target: Apoptosis Inhibitor 5 (API5)

Alternative Name: API5 (API5 Products)

Synonyms: AAC-11 antibody, AAC11 antibody, AI196452 antibody, api5l1 antibody, sr:nyz021 antibody, zgc:66211 antibody, wu:fb26c03 antibody, API-5B antibody, aac-11 antibody, aac11 antibody, api5 antibody, API-5A antibody, api5-A antibody, MGC83290 antibody, API5 antibody, apoptosis inhibitor 5 antibody, apoptosis inhibitor 5 S homeolog antibody, apoptosis inhibitor 5 L homeolog antibody, API5 antibody, Api5 antibody, api5 antibody, api5.S antibody, api5.L antibody

Background: Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death(apoptosis). Apoptosis inhibitor 5(API5) is an antiapoptotic factor which may have a role in protein assembly. The API5 gene to chromosome 11p12 based on an alignment of the API5 sequence with the genomic sequence It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.

Synonyms: AAC 11 antibody|AAC-11 antibody|AAC11 antibody|Antiapoptosis clone 11 protein antibody|API 5 antibody|API-5 antibody|API5 antibody|API5 like 1 antibody|API5_HUMAN antibody|API5L1 antibody|Apoptosis inhibitor 5 antibody|Cell migration inducing gene 8 protein antibody|Cell migration-inducing gene 8 protein antibody|Fibroblast growth factor 2 interacting factor 2 antibody|Fibroblast growth factor 2-interacting factor antibody|FIF antibody|MIG 8 antibody|MIG8 antibody|Migration inducing protein MIG8 antibody|Protein XAGL antibody

Pathways: Growth Factor Binding