S100B antibody (C-Term)

Catalog No. ABIN3044359

Product Details anti-S100B Antibody

Target: S100B (S100 Calcium Binding Protein B (S100B))

Binding Specificity: AA 64-78, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S100B antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Protein S100-B(S100B) detection. Tested with WB in Human,Mouse,Rat.

Sequence: DGDGECDFQE FMAFV

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Protein S100-B(S100B) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: S100 calcium binding protein B
Protein Name: Protein S100-B

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human S100 beta(64-78aa DGDGECDFQEFMAFV), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-S100B antibody (ABIN3044359)

Liu, Lv, Zhu, Wu, Zhang, Xu, Zheng, Zhao: "Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo." in: Scientific reports, Vol. 7, pp. 39869, (2018) (PubMed).

Sun, Li, Ma, Feng, Song, Lv, He: "Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-κB signaling in an IL-6-dependent manner." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 231, (2018) (PubMed).

Xu, Wu, Zhang, Lv, Zheng, Zhao: "Pro‑neurogenic effects of andrographolide on RSC96 Schwann cells in vitro." in: Molecular medicine reports, Vol. 14, Issue 4, pp. 3573-80, (2017) (PubMed).

Zuo, Wu, Zhang, Lv, Xu, Jiang, Zheng, Zhao: "Baicalin promotes the viability of Schwann cells in vitro by regulating neurotrophic factors." in: Experimental and therapeutic medicine, Vol. 14, Issue 1, pp. 507-514, (2017) (PubMed).

Wang, Tian, Xiao, Zhang, Sun, Tang: "A metanephric adenoma of the kidney associated with polycythemia: A case report." in: Oncology letters, Vol. 11, Issue 1, pp. 352-354, (2016) (PubMed).

Zhang, Zhao, Qu, Che, Lin, Jiang, Zhao, Wang: "Expression of S100B during the innate immune of corneal epithelium against fungi invasion." in: International journal of ophthalmology, Vol. 9, Issue 2, pp. 191-7, (2016) (PubMed).

Liu, Zhang, Zhao, Cui, Cao, Guo: "Effects of hypothermia on S100B and glial fibrillary acidic protein in asphyxia rats after cardiopulmonary resuscitation." in: Cell biochemistry and biophysics, Vol. 71, Issue 1, pp. 401-6, (2015) (PubMed).

Sun, Li, Ma, Liu, Xu, Ye, Hou, Wang, Li, Jiang: "The predictive value and potential mechanisms of miRNA-328 and miRNA-378 for brain metastases in operable and advanced non-small-cell lung cancer." in: Japanese journal of clinical oncology, Vol. 45, Issue 5, pp. 464-73, (2015) (PubMed).

Chen, Zhang, Liu, He, Zhou, Shao, Sun, Cao, Gong, Jiang: "A fibrin matrix promotes the differentiation of EMSCs isolated from nasal respiratory mucosa to myelinating phenotypical Schwann-like cells." in: Molecules and cells, Vol. 38, Issue 3, pp. 221-8, (2015) (PubMed).

Tang, Li, Cheng, Huang, Chen: "Effects of diethylstilbestrol on the proliferation and tyrosinase activity of cultured human melanocytes." in: Biomedical reports, Vol. 3, Issue 4, pp. 499-502, (2015) (PubMed).

Wülfing, Günther: "Dendritic cells and macrophages neurally hard-wired in the lymph node." in: Scientific reports, Vol. 5, pp. 16866, (2015) (PubMed).

Jin, Chen, Liu, Liang, Zhang, Zhang: "The expression of endothelial barrier antigen (EBA) and S100B in the rat parietal cortex following brain irradiation." in: Brain research, Vol. 1558, pp. 84-9, (2014) (PubMed).

Tang, Li, Cheng, Jing: "Primary culture of human face skin melanocytes for the study of hyperpigmentation." in: Cytotechnology, Vol. 66, Issue 6, pp. 891-8, (2014) (PubMed).

Lai, Wang, Ling, Wu, Zeng: "Graft of a tissue-engineered neural scaffold serves as a promising strategy to restore myelination after rat spinal cord transection." in: Stem cells and development, Vol. 23, Issue 8, pp. 910-21, (2014) (PubMed).

Zhao, He, Zhang, Tian, Zhao, Yu, Bai: "Electric stimulation and decimeter wave therapy improve the recovery of injured sciatic nerves." in: Neural regeneration research, Vol. 8, Issue 21, pp. 1974-84, (2014) (PubMed).

Qu, Liang, Gu, Liu: "Quercetin alleviates high glucose-induced Schwann cell damage by autophagy." in: Neural regeneration research, Vol. 9, Issue 12, pp. 1195-203, (2014) (PubMed).

Jiang, Qu, Han, Liu, Zhang: "Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia." in: International journal of molecular medicine, Vol. 30, Issue 3, pp. 480-6, (2012) (PubMed).

Target Details for S100B

Target: S100B (S100 Calcium Binding Protein B (S100B))

Alternative Name: S100B (S100B Products)

Synonyms: NEF antibody, S100 antibody, S100-B antibody, S100beta antibody, S100P antibody, AI850290 antibody, Bpb antibody, si:dkey-110p14.2 antibody, wu:fq18b10 antibody, zgc:92776 antibody, S100 calcium binding protein B antibody, S100 protein, beta polypeptide, neural antibody, S100 calcium binding protein, beta (neural) antibody, S100B antibody, S100b antibody, s100b antibody

Background: S100 calcium binding protein B or S100B is a protein of the S-100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21, however, this gene is located at 21q22.3. S100B is a glial-derived protein that is a well-established biomarker for severity of neurological injury and prognosis for recovery. S100 beta is a calcium-binding protein that is expressed at high levels in brain primarily by astrocytes. Addition of the disulfide-bonded dimeric form of S100 beta to primary neuronal and glial cultures and established cell lines induces axonal extension and alterations in astrocyte proliferation and phenotype, but evidence that S100 beta exerts the same effects in vivo has not been presented. Reeves et al.(1994) demonstrated that the same effects of the S100B protein are exerted in vivo. They found that both astrocytosis and neurite proliferation occurred in transgenic mice expressing elevated levels of S100b. They suggested that these transgenic mice represent a useful model for studies of the role of S100B in glial-neuronal interactions in normal development and function of the brain and for analyzing the significance of elevated levels of the protein in Down syndrome and Alzheimer disease.

Synonyms: NEF antibody|Protein S100 B antibody|Protein S100-B antibody|S 100 calcium binding protein beta chain antibody|S 100 protein beta chain antibody|S 100 protein beta subunit antibody|S-100 protein beta chain antibody|S-100 protein subunit beta antibody|S100 antibody|S100 calcium binding protein B antibody|S100 calcium binding protein beta(neural) antibody|S100 calcium binding protein beta chain antibody|S100 calcium-binding protein B antibody|S100 protein beta chain antibody|S100B antibody|S100B_HUMAN antibody|S100beta antibody

UniProt: P04271

Pathways: Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins