Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 393-412), (C-Term) antibody

Details for Product No. ABIN3044397, Supplier: Log in to see
Antigen
  • LOC100136415
  • 4933429D07Rik
  • AW742785
  • Nr1c1
  • PPAR-alpha
  • PPARalpha
  • Ppar
  • PPAR
  • NR1C1
  • nr1c1
  • hPPAR
  • xPPARalpha
  • xppara
  • PPARALPHA
  • peroxisome proliferator-activated receptor alpha
  • peroxisome proliferative activated receptor, alpha
  • peroxisome proliferator activated receptor alpha
  • LOC100136415
  • PPAR-ALPHA
  • Ppara
  • PPAR ALPHA
  • ppara
  • PPARA
Alternatives
anti-Rat (Rattus) Peroxisome Proliferator-Activated Receptor alpha antibody for Chromatin Immunoprecipitation
Epitope
AA 393-412, C-Term
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17
16
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15
13
9
7
5
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4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
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150
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25
17
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9
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3
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2
2
1
1
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Host
Rabbit
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102
Clonality
Polyclonal
Conjugate
Un-conjugated
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5
3
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3
3
3
3
3
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2
2
2
2
2
2
2
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2
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
211
104
97
75
59
46
31
26
14
6
5
4
3
3
3
2
2
1
Options
Supplier
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Available images

'Independent Validation' Badge
Antigen PPARA
Method validated Immunohistochemistry
Positive Control liver
Notes Passed. ABIN3044397 staining is consistent with RNA-seq data and published literature.
Primary Antibody ABIN3044397
Secondary Antibody HRP polymer (ThermoFisher Scientific, TL-125-PH)
Protocol
  • Sample preparation:
    • Fixation and TMA preparation according to PMID: 22688270.
    • Cut 4µm TMA sections using a waterfall microtome (ThermoFisher Scientific, HM355S).
    • Dry sections ON at RT and then back them for 12-24h at 50°C.
  • Deparaffinization and rehydration in xylene and graded alcohol series:
    • xylene twice for 3min.
    • 100% ethanol for 1min.
    • 95% ethanol for 1min.
    • 0.3% H2O2 in 95% ethanol for 5min to block endogenous peroxidase.
    • 70% ethanol for 1min.
    • 50% ethanol for 1min.
    • distilled H2O for 2min.
  • Antigen Retrieval by Heat Induced Epitope Retrieval (HIER):
    • Boil the sections in Lab Vision Citrate buffer pH6.0 (ThermoFisher Scientific, TA-250-PM1X) for 4min at 125°C in a decloaking chamber (Biocare Medical).
    • Allow slides to cool down to 90°C in the decloaking chamber.
  • Immunostaining in a Lab Vision Autostainer 480 (ThermorFisher Scientific) at RT with 300µl of reagents for each step:
    • Rinse sections in Lab Vision wash buffer with extra tween added to a final concentration of 0.2% (ThermoFisher Scientific, TA-999-TT and TA-125-TW) wash buffer.
    • Block sections with Lab Vision Ultra V-Block (ThermoFisher Scientific, TA-125-UB) for 5min.
    • Rinse sections 2x in wash buffer.
    • Incubate sections with primary rabbit anti-PPARA antibody (antibodies-online, ABIN3044397) diluted 1:1100 for 30min.
    • Rinse sections 3x in wash buffer.
    • Incubate sections with labeled HRP polymer (ThermoFisher Scientific, TL-125-PH) for 30min.
    • Rinse sections 2x in wash buffer.
    • Develop in DAB Quanto solution (ThermoFisher Scientific, TL-125-QHDX) for 5min.
    • Rinse sections in distilled H2O.
  • Washing, counterstaining, and coverslipping in Autostainer XL (Leica Biosystems, ST5010) at RT:
    • Counterstaining in Mayer’s hematoxylin plus (HistoLab, 01820) for 7.5min.
    • Rinse sections in tap water for 5min.
    • Rinse sections in lithium carbonate water diluted 1:5 for 5min.
    • Dehydration in graded ethanol series and Neo-Clear (Merck Millipore, 109843) according to the manufacturer recommendations.
    • Automated mounting (Leica Biosystems, CV5030) of coverslip with Pertex (Histolab, 00871).
  • Microscopy:
    • Image acquisition on a Leica Aperio AT2 and Aperio Scanscope AT at 20x magnification.
Experimental Notes Nuclear staining of PPARA with ABIN3044397 is observed in all tissues, consistent with RNA-seq data and supported by published work. ABIN3044397 does show also show some cytoplasmic and membrane staining but significantly weaker than nuclear staining.
Validation Images
Immunohistochemistry validation image for anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 393-412), (C-Term) antibody (ABIN3044397) Staining of human liver section in a tissue microarray with ABIN3044397. See the prot...
Purpose Rabbit IgG polyclonal antibody for Peroxisome proliferator-activated receptor alpha(PPARA) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of mouse PPAR alpha(393-412aa NIGYIEKLQEGIVHVLKLHL), different from the human sequence by four amino acids.
Sequence NIGYIEKLQE GIVHVLKLHL
Isotype IgG
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Peroxisome proliferator-activated receptor alpha(PPARA) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: peroxisome proliferator-activated receptor alpha
Protein Name: Peroxisome proliferator-activated receptor alpha(PPAR-alpha)
Purification Immunogen affinity purified.
Alternative Name PPARA (PPARA Antibody Abstract)
Background Peroxisome proliferator-activated receptor alpha(PPAR-alpha), also known as NR1C1(nuclear receptor subfamily 1, group C, member 1), is a nuclear receptor protein that in humans is encoded by the PPARA gene. PPARA gene spans 83.7 kb and contains 8 exons. And the PPAR gene is mapped to chromosome 22q12-q13.1. Sher et al.(1993) cloned a cDNA for human peroxisome proliferator-activated receptor from a human liver cDNA library. The PPAR cDNA exhibited 85 % and 91 % DNA and deduced amino acid sequence identity, respectively, with mouse PPAR. PPAR-alpha is a transcription factor and a major regulator of lipid metabolism in the liver.

Synonyms: hPPAR antibody|MGC2237 antibody|MGC2452 antibody|NR1C1 antibody|Nuclear receptor subfamily 1 group C member 1 antibody|OTTHUMP00000197740 antibody|OTTHUMP00000197741 antibody|Peroxisome Proliferator Activated Receptor alpha antibody|Peroxisome proliferator-activated receptor alpha antibody|PPAR antibody|PPAR-alpha antibody|PPARA antibody|PPARA_HUMAN antibody|PPARalpha antibody
UniProt P23204
Pathways Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 393-412), (C-Term) antibody (ABIN3044397) Anti-PPAR alpha antibody, IHC(P) IHC(P): Human Mammary Cancer Tissue
Immunohistochemistry (IHC) image for anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 393-412), (C-Term) antibody (ABIN3044397) Anti-PPAR alpha antibody, IHC(P) IHC(P): Human Mammary Cancer Tissue
Western Blotting (WB) image for anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 393-412), (C-Term) antibody (ABIN3044397) Lane 8: SW620 Cell Lysate
Immunohistochemistry (IHC) image for anti-Peroxisome Proliferator-Activated Receptor alpha (PPARA) (AA 393-412), (C-Term) antibody (ABIN3044397) Anti-PPAR alpha antibody, IHC(P) IHC(P): Mouse Intestine Tissue
Product cited in: Zhou, Zhang, Xu, Wang: "Curcumin ameliorates renal fibrosis by inhibiting local fibroblast proliferation and extracellular matrix deposition." in: Journal of pharmacological sciences, Vol. 126, Issue 4, pp. 344-50, 2015 (PubMed).

Li, Chen, Peng, Zhou, Fang: "Pulsed electromagnetic fields protect the balance between adipogenesis and osteogenesis on steroid-induced osteonecrosis of femoral head at the pre-collapse stage in rats." in: Bioelectromagnetics, Vol. 35, Issue 3, pp. 170-80, 2014 (PubMed).

Shi, Zhang, Zhang, Zhang, Wu, Cheng, Li: "Chronic exposure to contaminated drinking water stimulates PPAR expression in mice livers." in: Chemosphere, Vol. 88, Issue 4, pp. 407-12, 2012 (PubMed).