CCR10 antibody (C-Term)

Catalog No. ABIN3044415

Product Details anti-CCR10 Antibody

Target: CCR10 (Chemokine (C-C Motif) Receptor 10 (CCR10))

Binding Specificity: AA 312-327, C-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCR10 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for C-C chemokine receptor type 10(CCR10) detection. Tested with WB in Human,Mouse,Rat.

Sequence: FLGLRFRQDL RRLLRG

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for C-C chemokine receptor type 10(CCR10) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: chemokine(C-C motif) receptor 10
Protein Name: C-C chemokine receptor type 10(C-C CKR-10/CC-CKR-10/CCR-10)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GPR2/CCR10(312-327aa FLGLRFRQDLRRLLRG), different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CCR10

Target: CCR10 (Chemokine (C-C Motif) Receptor 10 (CCR10))

Alternative Name: CCR10 (CCR10 Products)

Synonyms: GPR2 antibody, Cmkbr9 antibody, Gpr2 antibody, zgc:91924 antibody, CCR10 antibody, C-C motif chemokine receptor 10 antibody, chemokine (C-C motif) receptor 10 antibody, CCR10 antibody, Ccr10 antibody, ccr10 antibody

Background: CCR10, C-C chemokine receptor type 10 is a protein that in humans is encoded by the CCR10 gene. CCR10 is constitutively expressed in skin melanocytes, fibroblasts, and microvascular endothelial cells, and that it is upregulated by IL1B and TNF. Expression was also detected in T cells, Langerhans cells, and peripheral blood mononuclear cells but not in dendritic cells.By fluorescence in situ hybridization, the GPR2 gene is mapped to 17q21.1-q21.3. CCR10 is the receptor for CCL27. CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation. expression of CCL27 in normal keratinocytes and its strong upregulation in skin lesions of atopic dermatitis, contact dermatitis, and psoriasis patients. CCR10+ T lymphocytes were detected in lesional but not normal skin of these patients. Flow cytometric analysis showed that CCL27 binds extracellular matrix components and dermal microvascular endothelial cells and fibroblasts and mediates adhesion and transendothelial migration of CCR10+ circulating leukocytes. CCR10 is predominantly expressed on CD4+CLA+(cutaneous lymphocyte antigen), rather than CD8+, circulating T cells. RT-PCR, confocal microscopy, and ELISA analysis indicated that keratinocytes exposed to TNF or IL1B but not to IL4 or IFNG in vitro express increased CCL27.

Synonyms: C C chemokine receptor type 10 antibody|C C CKR 10 antibody|C-C chemokine receptor type 10 antibody|C-C CKR-10 antibody|CC chemokine receptor 10 antibody|CC CKR 10 antibody|CC-CKR-10 antibody|CCR 10 antibody|CCR-10 antibody|CCR10 antibody|CCR10_HUMAN antibody|Chemokine(C C motif) receptor 10 antibody|G protein coupled receptor 2 antibody|G protein-coupled receptor 2 antibody|G-protein coupled receptor 2 antibody|GPR 2 antibody|GPR2 antibody

UniProt: P46092