Fascin antibody (N-Term)

Catalog No. ABIN3044444

Product Details anti-Fascin Antibody

Target: Fascin (FSCN1)

Binding Specificity: AA 97-113, N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fascin antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Fascin(FSCN1) detection. Tested with WB in Human,Mouse,Rat.

Sequence: DDGRWSLQSE AHRRYFG

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Fascin(FSCN1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: fascin homolog 1, actin-bundling protein(Strongylocentrotus purpuratus)
Protein Name: Fascin

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Fascin(97-113aa DDGRWSLQSEAHRRYFG), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-Fascin antibody (ABIN3044444)

Jiang, Wang, Chen: "Overexpression of FOXM1 is associated with metastases of nasopharyngeal carcinoma." in: Upsala journal of medical sciences, Vol. 119, Issue 4, pp. 324-32, (2014) (PubMed).

Target Details for Fascin

Target: Fascin (FSCN1)

Alternative Name: FSCN1 (FSCN1 Products)

Synonyms: CG15331 antibody, CG1536 antibody, CG32858 antibody, Dmel\\CG32858 antibody, SN antibody, Sn antibody, fs(1)A1057 antibody, fs(1)K1421 antibody, fs(1)K418 antibody, fs(1)K473 antibody, fs(1)K743 antibody, fs(1)M45 antibody, p55 antibody, snl antibody, MGC75811 antibody, fscn1 antibody, sb:cb589 antibody, zgc:153632 antibody, AI663989 antibody, Fan1 antibody, fascin-1 antibody, Fascin antibody, fscn antibody, FAN1 antibody, HSN antibody, SNL antibody, fascin antibody, singed antibody, fascin actin-bundling protein 1 antibody, fascin actin-bundling protein 1a antibody, fascin actin-bundling protein 1 L homeolog antibody, sn antibody, fscn1 antibody, fscn1a antibody, Fscn1 antibody, fscn1.L antibody, FSCN1 antibody

Background: Fascin is a actin cross-linking protein. The Fascin gene contains 5 exons and spans 7 kb. It is a 54-58 kilodalton monomeric actin filament bundling protein originally isolated from sea urchin egg but also found in Drosophila and vertebrates, including humans. Fascin(from the Latin for bundle) is spaced at 11 nanometre intervals along the filament. The bundles in cross section are seen to be hexagonally packed, and the longitudinal spacing is compatible with a model where fascin cross-links at alternating 4 and 5 actins. It is calcium insensitive and monomeric. Fascin binds beta-catenin, and colocalizes with it at the leading edges and borders of epithelial and endothelial cells. The role of Fascin in regulating cytoskeletal structures for the maintenance of cell adhesion, coordinating motility and invasion through interactions with signalling pathways is an active area of research especially from the cancer biology perspective. Abnormal fascin expression or function has been implicated in breast cancer, colon cancer, esophageal squamous cell carcinoma, gallbladder cancer and prostate cancer.

Synonyms: 55 kDa actin bundling protein antibody|55 kDa actin-bundling protein antibody|Actin bundling protein antibody|actin bundling protein, 55-KD antibody|FAN 1 antibody|FAN1 antibody|Fascin 1 antibody|Fascin antibody|Fascin homolog 1 actin bundling protein(Strongylocentrotus purpuratus) antibody|Fascin homolog 1 antibody|Fascin, sea urchin, homolog of, 1 antibody|Fascin1 antibody|FLJ38511 antibody|FSCN 1 antibody|FSCN1 antibody|FSCN1_HUMAN antibody|HSN antibody|p55 antibody|Singed(Drosophila) like(sea urchin fascin homolog like) antibody|Singed drosophila homolog like antibody|Singed like(fascin homolog sea urchin)(Drosophila) antibody|Singed like(fascin homolog sea urchin) antibody|Singed like protein antibody|Singed, drosophila, homolog of antibody|Singed-like protein antibody|SNL antibody|Strongylocentrotus purpuratus antibody

UniProt: Q16658