EGF antibody (AA 977-1029)

Catalog No. ABIN3044517

Product Details anti-EGF Antibody

Target: EGF (Epidermal Growth Factor (EGF))

Binding Specificity: AA 977-1029

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Rabbit IgG polyclonal antibody for Pro-epidermal growth factor(EGF) detection. Tested with WB, ELISA in Mouse.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Pro-epidermal growth factor(EGF) detection. Tested with WB, ELISA in Mouse.
Gene Name: epidermal growth factor
Protein Name: Pro-epidermal growth factor

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse EGF recombinant protein (Position: N977-R1029). Mouse EGF shares 69.8% and 77.4% amino acid (aa) sequence identity with human and rat EGF.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse

Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-EGF antibody (ABIN3044517)

Wang, Li, Fu, Li, Yang, Zhang, Zhu, Yang, Gu, Xing, Zhang: "Exemestane Attenuates Hepatic Fibrosis in Rats by Inhibiting Activation of Hepatic Stellate Cells and Promoting the Secretion of Interleukin 10." in: Journal of immunology research, Vol. 2017, pp. 3072745, (2018) (PubMed).

Target Details for EGF

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF (EGF Products)

Synonyms: HOMG4 antibody, URG antibody, AI790464 antibody, CEGF antibody, epidermal growth factor antibody, pro-epidermal growth factor antibody, EGF antibody, egf antibody, CpipJ_CPIJ020278 antibody, Egf antibody

Background: EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Synonyms: Beta urogastrone antibody|EGF antibody|Egf antibody|EGF_HUMAN antibody|Epidermal Growth Factor antibody|Epidermal Growth Factor antibody|HOMG4 antibody|OTTHUMP00000219721 antibody|OTTHUMP00000219722 antibody|Pro epidermal growth factor antibody|URG antibody| Urogastrone antibody

Gene ID: 13645

UniProt: P01132

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation