NUCB2 antibody (AA 25-106)

Catalog No. ABIN3044531

Product Details anti-NUCB2 Antibody

Target: NUCB2 (Nucleobindin 2 (NUCB2))

Binding Specificity: AA 25-106

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUCB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Nucleobindin-2(NUCB2) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Nucleobindin-2(NUCB2) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.
Gene Name: nucleobindin 2
Protein Name: Nucleobindin-2

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse Nucleobindin 2 recombinant protein (Position: V25-L106). Mouse Nucleobindin 2 shares 85.4% and 97.6% amino acid (aa) sequence identity with human and rat Nucleobindin 2, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for NUCB2

Target: NUCB2 (Nucleobindin 2 (NUCB2))

Alternative Name: NUCB2 (NUCB2 Products)

Synonyms: nefa antibody, MGC97715 antibody, NUCB2 antibody, nucleobindin-2 antibody, NEFA antibody, AI607786 antibody, Calnuc antibody, Nefa antibody, Nesfatin-1 antibody, p54 antibody, nucleobindin 2 antibody, nucleobindin 2 L homeolog antibody, nucb2 antibody, NUCB2 antibody, Nucb2 antibody, nucb2.L antibody

Background: Nucleobindin-2 is a calcium-binding EF-hand protein that in humans is encoded by the NUCB2 gene. By exon trapping and sequence analysis, the NUCB2 sequence has been identified on chromosome 11p15-p14. This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells.

Synonyms: DNA binding protein NEFA antibody|DNA-binding protein NEFA antibody|Gastric cancer antigen Zg4 antibody|NEFA antibody|Nefastin 1 antibody|Nefastin-1 antibody|Nefastin1 antibody|Nucb2 antibody|NUCB2_HUMAN antibody|Nucleobindin-2 antibody|Nucleobinding 2 antibody| Prepronefastin antibody|Prepronesfatin antibody

Gene ID: 53322

UniProt: P81117