SKAP1 antibody (Middle Region)

Catalog No. ABIN3044545

Product Details anti-SKAP1 Antibody

Target: SKAP1 (Src Kinase Associated phosphoprotein 1 (SKAP1))

Binding Specificity: AA 163-189, Middle Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Src kinase-associated phosphoprotein 1(SKAP1) detection. Tested with WB, IHC-P in Human,Rat.

Sequence: RMAPHLRRDS KKESCFELTS QDRRSYE

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Src kinase-associated phosphoprotein 1(SKAP1) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: src kinase associated phosphoprotein 1
Protein Name: Src kinase-associated phosphoprotein 1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SKAP55 (163-189aa RMAPHLRRDSKKESCFELTSQDRRSYE), different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for SKAP1

Target: SKAP1 (Src Kinase Associated phosphoprotein 1 (SKAP1))

Alternative Name: SKAP1 (SKAP1 Products)

Synonyms: 1700091G21Rik antibody, Scap1 antibody, Skap-55 antibody, SCAP1 antibody, SKAP55 antibody, Skap55 antibody, src family associated phosphoprotein 1 antibody, src kinase associated phosphoprotein 1 antibody, Skap1 antibody, SKAP1 antibody

Background: Src kinase-associated phosphoprotein 1 is an adapter protein that in humans is encoded by the SKAP1 gene. This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins.

Synonyms: pp55 antibody|SCAP 1 antibody|SCAP1 antibody|SKAP 1 antibody|SKAP 55 antibody|SKAP-55 antibody|Skap1 antibody|SKAP1_HUMAN antibody| SKAP55 adaptor protein antibody|SRC family associated phosphoprotein 1 antibody|Src family-associated phosphoprotein 1 antibody|Src kinase associated phosphoprotein 1 antibody|SRC kinase associated phosphoprotein 55 kD antibody|Src kinase associated phosphoprotein of 55 kDa antibody|Src kinase-associated phosphoprotein 1 antibody|Src kinase-associated phosphoprotein of 55 kDa antibody

Gene ID: 8631