SMURF1 antibody (Middle Region)

Catalog No. ABIN3044547

Product Details anti-SMURF1 Antibody

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Binding Specificity: AA 326-351, Middle Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMURF1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase SMURF1(SMURF1) detection. Tested with WB in Human,Rat.

Sequence: DHNNRTTQFT DPRLHHIMNH QCQLKE

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase SMURF1(SMURF1) detection. Tested with WB in Human,Rat.
Gene Name: SMAD specific E3 ubiquitin protein ligase 1
Protein Name: E3 ubiquitin-protein ligase SMURF1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SMURF1 (326-351aa DHNNRTTQFTDPRLHHIMNHQCQLKE), identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for SMURF1

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Alternative Name: SMURF1 (SMURF1 Products)

Synonyms: 4930431E10Rik antibody, mKIAA1625 antibody, RGD1309707 antibody, SMAD specific E3 ubiquitin protein ligase 1 antibody, E3 ubiquitin-protein ligase SMURF1 antibody, SMAD specific E3 ubiquitin protein ligase 1 L homeolog antibody, SMURF1 antibody, LOC100540915 antibody, smurf1 antibody, Smurf1 antibody, smurf1.L antibody

Background: E3 ubiquitin-protein ligase SMURF1 is an enzyme that in humans is encoded by the SMURF1 gene. It is mapped to 7q22.1. This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.

Synonyms: E3 ubiquitin-protein ligase SMURF1 antibody|hSMURF1 antibody|KIAA1625 antibody|Smad specific E3 ubiquitin ligase 1 antibody|SMAD specific E3 ubiquitin protein ligase 1 antibody|Smad ubiquitination regulatory factor 1 antibody|SMAD-specific E3 ubiquitin-protein ligase 1 antibody|SMUF1_HUMAN antibody|SMURF 1 antibody|smurf1 antibody

Gene ID: 57154

Pathways: Regulation of Cell Size