SMYD3 antibody (C-Term)

Catalog No. ABIN3044548

Product Details anti-SMYD3 Antibody

Target: SMYD3 (SET and MYND Domain Containing 3 (SMYD3))

Binding Specificity: AA 388-428, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMYD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Histone-lysine N-methyltransferase SMYD3(SMYD3) detection. Tested with WB, IHC-P in Human.

Sequence: QAMKNLRLAF DIMRVTHGRE HSLIEDLILL LEECDANIRA S

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Histone-lysine N-methyltransferase SMYD3(SMYD3) detection. Tested with WB, IHC-P in Human.
Gene Name: SET and MYND domain containing 3
Protein Name: Histone-lysine N-methyltransferase SMYD3

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SMYD3 (388-428aa QAMKNLRLAFDIMRVTHGREHSLIEDLILLLEECDANIRAS), different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for SMYD3

Target: SMYD3 (SET and MYND Domain Containing 3 (SMYD3))

Alternative Name: SMYD3 (SMYD3 Products)

Background: SET and MYND domain-containing protein 3 is a protein that in humans is encoded by the SMYD3 gene. The International Radiation Hybrid Mapping Consortium mapped the SMYD3 gene to chromosome 1. This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms: bA74P14.1 (novel protein) antibody|bA74P14.1 antibody|FLJ21080 antibody|histone lysine N methyltransferase SMYD3 antibody|KMT3E antibody|MGC104324 antibody|SET and MYND domain containing 3 antibody|SET and MYND domain containing protein 3 antibody|SET and MYND domain-containing protein 3 antibody|SMYD 3 antibody|Smyd3 antibody|SMYD3 protein antibody|SMYD3_HUMAN antibody|Zinc finger MYND domain containing 1 antibody|Zinc finger MYND domain containing protein 1 antibody|Zinc finger MYND domain-containing protein 1 antibody|Zinc finger protein subfamily 3A MYND domain containing 1 antibody|Zinc finger protein, subfamily 3A (MYND domain containing), 1 antibody|ZMYND 1 antibody|ZMYND1 antibody|ZNFN3A1 antibody

Gene ID: 64754

UniProt: Q9H7B4