CD55 antibody (PE)

Catalog No. ABIN3044626

Product Details anti-CD55 Antibody (PE)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CD55 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: The anti-Mouse DAF monoclonal antibody detects DAF; one of several membrane proteins that prevent host cells from homologous complement attack. The two mouse DAF isoforms are effective against rat complement, but not against human and guinea pig complement. GPI-DAF is expressed only in testis. Both types of DAF transfectans avoided C3 deposition more successfully than non-transfectant cells.

Immunogen: GPI-DAF transfected CHO cells. Species

Clone: RIKO-3

Isotype: IgG

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis using less than or equal to 0,25 µg/10^6 cells

Comment:

R-Phycoerythrin (R-PE). Abs/Em: 545/575 nm.

Reagent Preparation:

1. Transfer the sample of blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add anti-mouse CD55 PE and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. CD55 recognizes the erythrocytes, so if you want a white blood cell labeling will be required prior lyse with ammonium chloride. 3. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55 (CD55 Products)

Synonyms: CR antibody, CROM antibody, DAF antibody, TC antibody, Daf antibody, Daf-GPI antibody, Daf1 antibody, GPI-DAF antibody, CD55 molecule (Cromer blood group) antibody, CD55 molecule, decay accelerating factor for complement antibody, CD55 antibody, Cd55 antibody

Background: CD55, also known as DAF (decay accelerating factor), is expressed on the surfaces of red blood cells, hematopoietic cells and many non-hemotopietic cells. There are two isoforms of mouse CD55, a GPI-anchored isoform and a transmembrane isoform. Both are recognized by RIKO-3. The function of CD55 is to protect damage by complement deposition

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity